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Neuroscience Neurotransmitter Amino Acids Glutamate

人EAAT2多肽(ab41752)

  • Datasheet
Reviews (1) Submit a question References (1)

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Human EAAT2 peptide (ab41752)

    Key features and details

    • Purity: > 90% n/a
    • Suitable for: Blocking

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    描述

    • 产品名称

      人EAAT2多肽
    • 纯度

      > 90 % n/a.

    • Accession

      P43004
    • 无动物成分

      No
    • 性质

      Synthetic
      • 种属

        Human

    相关产品

    • Corresponding Antibody

      • Anti-EAAT2 antibody (ab41621)

    技术指标

    Our Abpromise guarantee covers the use of ab41752 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Blocking - Blocking peptide for Anti-EAAT2 antibody (ab41621)

    • 形式

      Lyophilized
    • 补充说明

      Reconstitution instructions:

       -  First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.

      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.

      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.

      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.

      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    常规信息

    • 别名

      • EAA2_HUMAN
      • EAAT2
      • Excitatory amino acid transporter 2
      • Excitotoxic amino acid transporter 2
      • Glial high affinity glutamate transporter
      • GLT 1
      • GLT1
      • Glutamate aspartate transporter II
      • Glutamate transporter 1
      • Glutamate/aspartate transporter II
      • Slc1a2
      • Sodium dependent glutamate aspartate transporter 2
      • Sodium-dependent glutamate/aspartate transporter 2
      • solute carrier family 1 (glial high affinity glutamate transporter), member 2
      • Solute carrier family 1 glial high affinity glutamate transporter member 2
      • Solute carrier family 1 member 2
      see all
    • 功能

      Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
    • 序列相似性

      Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
    • 翻译后修饰

      Glycosylated.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P43004 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    图片

    • Human EAAT2 peptide (ab41752)
      Human EAAT2 peptide (ab41752)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab41752?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab41752 被引用在 1 文献中.

    • Potapenko ES  et al. Altered astrocyte glutamate transporter regulation of hypothalamic neurosecretory neurons in heart failure rats. Am J Physiol Regul Integr Comp Physiol 303:R291-300 (2012). PubMed: 22696576

    客户评价及客户问答

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    提交评价 提交问题

    Blocking - Blocking peptide for Anti-EAAT2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use
    Predicted band size would be 62 kDa.
    however observed band size for cow liver is less then 62 kDa.
    Loading amount of each lane : 60ug of cow liver
    1 lane: Fresh Primary antibody (ab41621)
    2 lane: Fresh Primary antibody with 45 min incubation at RT
    3 lane: only Secondary antibody (without primary antibody)
    4 lane: Primary antibody : Peptide = 1 : 0.01with 45 min incubation at RT
    5 lane: Primary antibody : Peptide = 1 : 0.001with 45 min incubation at RT
    6 lane: Primary antibody : Peptide = 1 : 0.0001with 45 min incubation at RT
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Kwangwon Son

    Verified customer

    提交于 Nov 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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