为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Tags & Cell Markers Epitope Tags HA Tag

HA tag多肽(ab13835)

  • Datasheet
Submit a review Q&A (3)References (4)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - HA tag peptide (ab13835)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking

    你可能会感兴趣

    一抗
    Product image
    Anti-HA tag antibody (ab13834)
    一抗
    Product image
    Anti-HA tag antibody [EPR22819-101] (ab236632)
    一抗
    Product image
    PE Anti-HA tag antibody [16B12] (ab72479)

    查看更多相关产品

    描述

    • 产品名称

      HA tag多肽
    • 纯度

      > 90 % HPLC.

    • Accession

      P03437
    • 无动物成分

      Yes
    • 性质

      Synthetic

      相关产品

      • Corresponding Antibody

        • Anti-HA tag antibody (ab13834)

      技术指标

      Our Abpromise guarantee covers the use of ab13835 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • 应用

        Blocking - Blocking peptide for Anti-HA tag antibody (ab13834)

      • 形式

        Lyophilized
      • 补充说明

        - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
        - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
        - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
        - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
        - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

      • Concentration information loading...

      制备和贮存

      • 稳定性和存储

        Shipped at 4°C. Store at -20°C.

        Information available upon request.

      常规信息

      • 别名

        • HA epitope tag
        • HA1
        • HA2
        • hemagglutinin
        • Hemagglutinin HA1 chain
        • Hemagglutinin HA2 chain
        see all
      • 相关性

        Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

      图片

      • Western blot - HA tag peptide (ab13835)
        Western blot - HA tag peptide (ab13835)
        Western blot using ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896). Lane 1: ab13834 Lane 2: ab13834 with blocking peptide ab13835. Primary: Rabbit polyclonal to HA tag (1/500) Secondary: Alexa Fluor 680 Goat anti Rabbit IgG (1/5000). Lysates at 10µg/lane.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (4)

      发表研究结果有使用 ab13835?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab13835 被引用在 4 文献中.

      • Marwarha G  et al. NF-κB Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression. Int J Mol Sci 24:N/A (2023). PubMed: 37047592
      • Marwarha G  et al. GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia. Int J Mol Sci 23:N/A (2022). PubMed: 36012628
      • Zhao Y  et al. Arabidopsis Duodecuple Mutant of PYL ABA Receptors Reveals PYL Repression of ABA-Independent SnRK2 Activity. Cell Rep 23:3340-3351.e5 (2018). PubMed: 29898403
      • Zhao Y  et al. ABA receptor PYL9 promotes drought resistance and leaf senescence. Proc Natl Acad Sci U S A 113:1949-54 (2016). PubMed: 26831097

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-3 of 3 Abreviews or Q&A

      Question


      I am attempting to find a product that would be used to create a standard curve for a quantitative western. My protein of interest has an HA-tag but cannot really be purified, would product ab13835 be suitable for creating a standard curve in a western?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 24 2012

      Answer

      Thank you for contacting Abcam regarding ab13835.


      This product is the HA peptide used as the immunogen to generate the antibody ab13834. Since it is only 9aa, it cannot be run on a gel to use as a positive control or standard. Instead, I would recommend a purified HA-tagged protein or a cell lysate containing an HA tagged protein, such as ab20896.


      I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

      Read More

      Abcam Scientific Support

      回复于 Sep 24 2012

      Question

      Dear Tech Support Team,

      Our customer wishes to use ab13835 in IP with anti HA from Santa Cruz SC-805. Kindly advise if ab13835 is suitable for this purpose. Will the mentioned above antibody recognize ab13835?

      Thanks in advance for your assistance and reply.

      Kind Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 27 2012

      Answer

      Thank you for contacting us.
      Yes, we expect that antibody to bind this peptide. The peptide ab13835 does contain the classical HA tag (amino acid 98 to 106 of the surface hemagglutinin glycoprotein of Human influenza virus). The cited antibody is directed against the HA tag and should therefore be able to recongize this peptide ab13835.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Mar 27 2012

      Question

      Thank you very much for your important information. I'm wondering do you have some experience of using this HA peptide (ab13835) for elution, such as the quantity needed for elution, etc? I did not find such information on the data sheet.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 24 2011

      Answer

      Here is a general protocol for eluting your HA-tagged protein with ab13835, our HA tag peptide: The peptide arrives at a 1mg/ml concentration. After the last wash, remove all of the wash buffer and add a sufficient volume of the peptide for your downstream use. For example, 30-50ul of the peptide might be a useful volume. Elute for at least 1 hour at 4 degrees C with gentle agitation. Pellet the beads and remove the supernatant. Confirm elution of target protein via coomassie staining and/or western blotting.

      Read More

      Abcam Scientific Support

      回复于 Jun 24 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2025 艾博抗(上海)贸易有限公司版权所有