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AB51992

EGFP蛋白(His tag)

EGFP protein (His tag)

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(1 Publication)

EGFP protein (His tag) is a recombinant Aequorea victoria Full Length protein, in the 2 to 238 aa range, with >=97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, western blot and conjugation assays. The predicted molecular weight of ab51992 protein is 32.7 kDa.

- ideal for use as a positive control
- Contains N-terminal and C-terminal His tags
- Available in different sizes to fit your experimental needs

See more GFP products

查看别名

Green fluorescent protein, GFP

关键信息

纯度

>97% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

His tag C-Terminus His tag N-Terminus

应用

Conjugation, SDS-PAGE, WB

applications

生物活性

No

访问

P42212

不含动物源

No

不含载体蛋白

No

种属

Aequorea victoria

复溶

Reconstitute in PBS

存储溶液

Constituents: PBS

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

  • 0
  • 0
Proteins & Peptides

AB199750

Recombinant mCherry protein (His tag)

  • 4
  • 1
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB171581

GFP ELISA Kit

SimpleStep

Sandwich ELISA - GFP ELISA Kit (AB171581)

  • 5
  • 3
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Conjugation": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Ensure the validity of your result using our EGFP protein (His tag) ab51992 as a control in SDS-PAGE and western blot.

This Enhanced GFP (eGFP) protein is suitable as control reagent for GFP expression studies. Other applications include: calibration of fluorometers and flow cytometers, fluorescence microscopy, and microinjection of GFP into cells and tissues, etc.

The excitation and emmission spectra for this ab51992 recombinant EGFP is Ex/Em = 488/509 nm.


Check out our protein gel staining guide for SDS-PAGE here

Check out our western blot protocol for more information here


This product is manufactured by BioVision, an Abcam company and was previously called 4999 Enhanced Green Fluorescent Protein (EGFP). 4999-100 is the same size as the 100 µg size of ab51992.
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMASVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKASMTGGQQMGRDPNSSSVDKLAAALEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"32.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Lyophilized
运输条件
Blue Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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常规信息

功能

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

序列相似性

Belongs to the GFP family.

翻译后修饰

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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产品实验方案

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靶点信息

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 19:5240-5252 PubMed39871506

2025

Large and Stable Nanopores Formed by Complement Component 9 for Characterizing Single Folded Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Wachara Chanakul,Anasua Mukhopadhyay,Saurabh Awasthi,Anna D Protopopova,Alessandro Ianiro,Michael Mayer
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com