Bovine Serum Albumin (nitrated ) (ab131379)
Key features and details
- Expression system: Native
- Purity: > 96% SDS-PAGE
- Suitable for: SDS-PAGE, WB
描述
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产品名称
Bovine Serum Albumin (nitrated )
参阅全部 Bovine Serum Albumin 蛋白酶 -
纯度
> 96 % SDS-PAGE. -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Cow -
序列
MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIA FSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCK VASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEF KADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGAC LLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAE FVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKE CCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFL GSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKL KHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVS RSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCC TESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQT ALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVV STQTALA -
预测分子量
69 kDa -
氨基酸
1 to 607
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab131379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
ab131379 was treated with peroxynitrite which modifies tyrosine by acting as an oxidant to produce 3-nitrotyrosine along the length of the Bovine Serum Albumin protein.
Product was previously marketed under the MitoSciences sub-brand.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.5
Constituents: 0.61% Tris HCl, 15% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
常规信息
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别名
- alb
- ALBU_HUMAN
- Albumin
see all - Information by UniProt
图片
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All lanes: Anti-Nitrotyrosine antibody (ab110282) 0.4 µg/mL.
Lane 1: Molecular weight marker
Lane 2: BSA 100 ng
Lane 3: BSA 25 ng
Lane 4: Nitrated BSA 100 ng
Lane 5: Nitrated BSA 50 ng
Lane 6: Nitrated BSA 25 ng
Lane 7: Nitrated BSA 12.5 ng
Lane 8: Nitrated BSA 6.25 ng
Lane 9: Nitrated BSA 3.125 ngSecondary antibody:
Goat polyclonal to Mouse IgG – Pre-Adsorbed (HRP) at 1/6000
developed using the ECL technique.Performed under reducing conditions.
Predicted band size: 69,293 Da
Observed band size: 69 kDaExposure time: 5 minutes
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Lane 1: Nitrated Bovine Serum Albumin protein (50 ng)
Lane 2: Molecular weight marker
Lane 3: Bovine Serum Albumin protein (50 ng)Gel: 4-20% acrylamide separation gel
Predicted band size: 69 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131379 尚未被引用在任何文献中。