AB51995

AGE-BSA

Name: AGE-BSA (ab51995)| Abcam中文官网
Brand: Abcam Limited
SKU: AB51995
Price: 4490 CNY
Availability: InStock

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(31 Publications)

AGE-BSA is modified bovine serum albumin (BSA) to create Advanced glycation end products (AGE)-BSA with >98% purity and suitable for SDS-PAGE. The predicted molecular weight of ab51995 protein is 66.5 kD.

- Save time and ensure accurate and specific results by using our AGE- BSA protein as a control
- Fluorescence of AGE-BSA: excitation/emission = 370/440 nm
- Cited in over 20 publications

查看别名

AGEs, Advanced glycation end products

1 Images

SDS-PAGE

Supplier Data

SDS-PAGE - AGE-BSA (AB51995)

4-20% SDS-PAGE of AGE-BSA : 10, 15 and 20 ug of AGE-BSA loaded in each lane under reducing conditions and stained with Coomassie Blue. AGE-BSA has a predicted MW of ~ 66.5 kDa

关键信息

纯度

>98% SDS-PAGE

表达系统

Native

应用

SDS-PAGE

applications

生物活性

不含动物源

不含载体蛋白

Yes

存储溶液

Constituents: PBS

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

The AGE modified BSA was produced by reacting BSA with glycolaldehyde under sterile conditions followed by extensive dialysis and purification steps.

ab51995 may precipitate during extended storage. Sonicate to solubilize the precipitates.

Fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex/Em = 370/440 nm.
Glycated BSA shows a 7000% increase in fluorescence in compared to control BSA.

Ensure the validity of your result using our AGE-BSA protein ab51995 as a positive control in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here

This product is manufactured by BioVision, an Abcam company and was previously called 2221 AGE-BSA. 2221-10 is the same size as the 10 mg size of ab51995.

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序列信息

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":"66.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"","expressionSystem":"Native","accessionNumber":null,"tags":[]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Advanced Glycation End-products (AGEs) are highly reactive compounds formed when reducing sugars non-enzymatically bind to proteins lipids or nucleic acids. They are often referred to as AGE products or AGE-BSA when in the form of bovine serum albumin (BSA). The molecular weight of AGE compounds can vary significantly due to the diversity of glycation products. AGEs express in various tissues but accumulate more in aging tissues and in certain pathological states. Detection of AGE products often utilizes AGE ELISA assays or fluorescence-based methods to measure AGE-BSA.

Biological function summary

AGE compounds affect cellular and tissue functions by altering structural proteins and increasing oxidative stress. While not a component of a specific complex AGEs catalyze cross-linking of proteins like collagen impacting tissue elasticity. The binding of AGEs to receptors such as RAGE (Receptor for Advanced Glycation End-products) triggers inflammatory pathways and oxidative stress. This interaction fuels the damaging effects of AGEs in biological systems which are assayed through AGE assays.

Pathways

AGE compounds influence several critical biological pathways particularly the NF-kB and MAPK pathways. These pathways mediate inflammation and cellular stress responses. The interaction with RAGE also affects signaling with related proteins like S100/calgranulins which further activate pro-inflammatory responses. The alteration of these pathways by AGE compounds highlights their significance in cellular dysfunction and age-related conditions.

AGE compounds play significant roles in the progression of diabetes and cardiovascular disease. In diabetes AGEs contribute to complications by modifying vascular structure and function where they link with proteins such as HbA1c in glycation. In cardiovascular disease AGE accumulation in vascular tissues enhances vascular stiffness and atherosclerotic lesion formation. The AGE-RAGE interaction perpetuates chronic inflammation further exacerbating these conditions.

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特殊说明

形式

Liquid

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常规信息

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information AGE

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文献 (31)

Recent publications for all applications. Explore the full list and refine your search

Inflammation : PubMed40021543

2025

Inhibition of Cullin3 Neddylation Alleviates Diabetic Retinopathy by Activating Nrf2 Signaling to Combat ROS-Induced Oxidative Stress and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqin Chen,Cong Liu,Jun Tong,Chang He,Xinru Ling,Jinjin Xiang,Chunyan Xue,Genhong Yao,Lingyun Sun,Zhenggao Xie

Stem cell research & therapy 15:431 PubMed39548506

2024

AGEs impair osteogenesis in orthodontic force-induced periodontal ligament stem cells through the KDM6B/Wnt self-reinforcing loop.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaohui Ying,Yujun Jiang,Changyun Sun,Yaoguang Zhang,Ruihan Gao,Hongrui Liu,Hongrui Liu,Jie Guo,Minqi Li

Virulence 15:2352476 PubMed38741276

2024

GlmS plays a key role in the virulence factor expression and biofilm formation ability of promoted by advanced glycation end products.

Applications

Unspecified application

Species

Unspecified reactive species

Lijia Ni,Rui Shen,Hua Luo,Xuexue Li,Xiaofan Zhang,Lisi Huang,Yawen Deng,Xiaoyan Liao,Yonglin Wu,Chaohui Duan,Xiaoying Xie

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308378 PubMed38483947

2024

Deficiency of Nuclear Receptor Coactivator 3 Aggravates Diabetic Kidney Disease by Impairing Podocyte Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Xie,Qian Yuan,Xinyi Cao,Yang Qiu,Jieyu Zeng,Yiling Cao,Yajuan Xie,Xianfang Meng,Kun Huang,Fan Yi,Chun Zhang

Frontiers in immunology 14:1264447 PubMed38022581

2023

SARS-CoV-2 N protein induced acute kidney injury in diabetic db/db mice is associated with a Mincle-dependent M1 macrophage activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wu,Wenbiao Wang,Liying Liang,Junzhe Chen,Sifan Sun,Biao Wei,Yu Zhong,Xiao-Ru Huang,Jian Liu,Xiaoqin Wang,Xueqing Yu,Hui-Yao Lan

Diabetes, metabolic syndrome and obesity : targets and therapy 16:3045-3056 PubMed37810573

2023

Advanced Glycation End Products Downregulate Connexin 43 and Connexin 40 in Diabetic Atrial Myocytes via the AMPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Huan-Huan Liu,Lei Zhang,Xiao-Lu Zhang,Jie Zhang,Feng Li,Ning Zhao,Zhi-Yuan Zhang,Qi Kong,Xiao-Yu Liu,Ying Wu,Zhi-Ming Yu,Ling-Ling Qian,Ru-Xing Wang

Diabetes & vascular disease research 20:14791641231197107 PubMed37592725

2023

Advanced glycation end products impair coronary artery BK channels via AMPK/Akt/FBXO32 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Li,Ling-Ling Qian,Ying Wu,Yu-Min Zhang,Shi-Peng Dang,Xiao-Yu Liu,Xu Tang,Cun-Yu Lu,Ru-Xing Wang

Environmental toxicology 38:2772-2782 PubMed37551785

2023

N6-methyladenosine methylation regulator RBM15 promotes the progression of diabetic nephropathy by regulating cell proliferation, inflammation, oxidative stress, and pyroptosis through activating the AGE-RAGE pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhang Qin,Suzhen Wu,Fengxia Zhang,Xueyan Zhou,Cong You,Fei Tan

Scientific reports 13:12609 PubMed37537205

2023

Increased dietary intake of ultraprocessed foods and mitochondrial metabolism alterations in pediatric obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Coppola,Lorella Paparo,Giovanna Trinchese,Andrea Margarita Rivieri,Antonio Masino,Anna Fiorenza De Giovanni Di Santa Severina,Mariapina Cerulo,Maria Escolino,Assunta Turco,Ciro Esposito,Maria Pina Mollica,Roberto Berni Canani

Nutrients 15: PubMed37432277

2023

Resveratrol Alleviates Diabetic Periodontitis-Induced Alveolar Osteocyte Ferroptosis Possibly via Regulation of SLC7A11/GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Zhijun Huang,Shuaifei Pan,Yuhui Feng,Haokun He,Shuguang Cheng,Lijing Wang,Liping Wang,Janak Lal Pathak

View all publications

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AGE-BSA

AGE-BSA

SDS-PAGE - AGE-BSA (AB51995)

关键信息

纯度

表达系统

标签

应用

生物活性

不含动物源

不含载体蛋白

存储溶液