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Accelerate immunometabolism flow discovery

Get deeper insights from rare cell populations with a unique portfolio of flow-validated antibodies to 90% of key metabolic enzymes. Drop-in ready for rapid panel expansion.

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Unlock immunometabolism analysis by flow cytometry with a unique portfolio of flow‑validated antibodies covering over 90% of key metabolic enzymes across glycolysis, TCA cycle, oxidative phosphorylation, protein and DNA metabolism and lipid metabolism. Designed specifically for flow, these reagents enable you to reveal previously inaccessible metabolic phenotypes in rare cell populations at true single‑cell resolution. Also known as met-flow, this approach allows you to study metabolism in different immune cell types, including T cells, B cells, macrophages, DCs and ILCs.

Our recombinant antibodies are knockout‑validated and tested in flow cytometry, including in primary cells such as PBMCs. With consistent lot‑to‑lot performance and no need for DIY conjugation, you can trust your data from the first experiment.

With multiple bright fluorophore options and drop‑in compatibility with existing panels, our immunometabolism flow antibodies make it easy to expand your flow panels without compromise so you can generate richer data and reduce optimisation cycles.

All clones react with human, mouse and rat samples unless otherwise indicated:

* reacts with human samples

** reacts with human and mouse samples

Target
Recommended clone
Available fluorescent conjugates
Description
GLUT1
EPR3915
SuperNova violet 428, Alexa Fluor® 647, APC, PE
Primary glucose transporter supporting increased energy demand during immune activation. Associated with metabolic reprogramming in inflammation and cancer.
HK1
EPR10134(B)
SNv428, AF® 488,  AF® 647, APC
Catalyzes the first committed step of glycolysis. Associated with increased glucose utilization in activated immune cells and metabolically stressed cells.
GAPDH
EPR16891
SNv428, AF® 488, AF® 647, PE
Core glycolytic enzyme commonly elevated during immune activation and proliferation. Used as an indicator of increased glycolytic flux.
PKM
EPR10138(B)
AF® 647, PE
Regulates the final ATP generating step of glycolysis. Associated with immune activation, inflammation, and cancer metabolism.
LDH
EP1566Y
AF® 488, APC, PE
Supports lactate production to sustain glycolysis under hypoxia or inflammation. Commonly associated with activated immune cells and tumor microenvironments.
GYS1
EP817Y*
AF® 488, AF® 647, PE
Enables glycogen synthesis as a glucose reserve. Implicated in metabolic flexibility during immune activation and nutrient stress.
HIF-1a
EP1215Y*
AF® 488
Central regulator of hypoxia induced metabolic adaptation. Associated with glycolytic gene expression in inflamed tissues and hypoxic immune niches.
Visfatin
EPR21980
AF® 488, AF® 647
Central regulator of hypoxia induced metabolic adaptation. Associated with glycolytic gene expression in inflamed tissues and hypoxic immune niches.
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Glycolysis
Target
Recommended clone
Available fluorescent conjugates
Description
CS
EPR8067
AF® 488,  AF® 647, APC, PE
Initiates the TCA cycle and is widely used as a marker of mitochondrial content. Reflects oxidative metabolic capacity.
IDH2
EPR7577
AF® 488,  AF® 647, APC, PE
Mitochondrial enzyme producing NADPH to support redox balance and biosynthesis. Implicated in cancer metabolism and metabolic stress responses.
OGDH
EPR27181-78
Conjugation-ready
Regulates carbon flow through the TCA cycle. Sensitive to mitochondrial activity and redox state.
SDHB
EPR13042(B)*
AF® 488, AF® 647, APC, PE
Links the TCA cycle to the electron transport chain (Complex II). Dysregulation is associated with altered respiration, inflammation, and tumor metabolism.
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TCA cycle
Target
Recommended clone
Available fluorescent conjugates
Description
Cyt c
7H8.2C12*
AF® 488, PE
Electron carrier within the respiratory chain. Redistribution is associated with mitochondrial dysfunction and apoptosis.
ATP5A
EPR13030(B)
AF® 488, PE
Core subunit of ATP synthase (Complex V). Reflects oxidative phosphorylation and ATP producing capacity.
AIF
E20
AF® 488, AF® 647
Mitochondrial protein involved in caspase-independent cell death. Associated with mitochondrial stress responses.
VDAC1/Porin + VDAC2
EPR10852(B)
AF® 488
Outer mitochondrial membrane channels regulating metabolite exchange. Common markers of mitochondrial mass.
MFN2
EPR19796*
AF® 488
Regulates mitochondrial fusion and network dynamics. Implicated in immune cell metabolism and stress adaptation.
mtTFA
18G102B2E11*
AF® 488, PE
Maintains mitochondrial DNA stability and transcription. Marker of mitochondrial biogenesis.
CPOX
EPR11927(B)**
AF® 647, PE
Enzyme in heme biosynthesis supporting respiratory complex function. Associated with mitochondrial metabolic competence.
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OXPHOS
Target
Recommended clone
Available fluorescent conjugates
Description
ASCT2/ SLC1A5
CAL33*
AF® 488,  AF® 647
Major glutamine transporter supporting nutrient uptake during immune activation. Implicated in mTOR signaling.
CD98
EPR27110-42*
APC, PE
Essential amino acid transport complex linked to nutrient sensing and growth signaling in immune cells.
GLS
EPR19525
Conjugation-ready
Initiates glutaminolysis by converting glutamine to glutamate. Supports TCA input in activated immune cells, redox balance, and cancer cell survival.
GLUD1+GLUD2
EPR11370
AF® 488,  AF® 647, APC, PE
Link amino acid catabolism to mitochondrial energy production. Support metabolic flexibility in immune and cancer cells.
ASS1
EPR12398
APC, PE
Regulates arginine biosynthesis and availability. Implicated in immune effector function.
CAD
EP710Y*
AF® 488,  AF® 647, APC, PE
Drives de novo pyrimidine synthesis required for DNA replication. Associated with proliferating immune cells.
DPD
EPR8811*
AF® 647
Controls pyrimidine catabolism and nucleotide turnover. Relevant to metabolic homeostasis and therapeutic response in cancer.
G6PD
EPR20668
SNv428, PE
Rate limiting enzyme of the pentose phosphate pathway. Central to NADPH production, redox control, and immune cell survival.
SHMT2/SHMT
EPR28607-51
Conjugation-ready
Mitochondrial one carbon metabolism enzyme supporting nucleotide synthesis and redox balance. Important in proliferating and metabolically stressed cells.
ENT1
SP120*
AF® 488,  AF® 647, APC, PE
Mediates bidirectional nucleoside uptake across the plasma membrane. Critical for immunometabolism by controlling adenosine availability, thereby influencing immune cell activation, proliferation, and inflammatory responses.
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Protein and DNA metabolism
Target
Recommended clone
Available fluorescent conjugates
Description
CD36 / FAT
EPR22509-40
AF® 488
Facilitates uptake of long chain fatty acids. Plays a central role in lipid‑driven immune regulation, inflammation, and metabolic reprogramming in cancer.
ACACA
EPR23235-47
Conjugation-ready
Rate‑limiting enzyme of fatty acid synthesis producing malonyl‑CoA. Regulates lipid biosynthesis during immune activation.
ACLY
EP704Y
AF® 488,  AF® 647, PE
Links mitochondrial metabolism to lipid biosynthesis by generating cytosolic acetyl‑CoA. Supports membrane production and growth in activated cells.
DGAT1
EPR13430-4*
Conjugation-ready
Catalyzes triglyceride synthesis and lipid droplet formation. Associated with metabolic stress adaptation.
CPT1A
EPR21843-71-2F
Conjugation-ready
Rate limiting enzyme controlling mitochondrial fatty acid oxidation. Implicated in immune differentiation and cancer metabolism.
ACADM / MCAD
EPR3708
AF® 488
Catalyzes mitochondrial β-oxidation of medium-chain fatty acids. Marker of FAO capacity.
FASN
EPR7465
AF® 488, APC, PE
Central enzyme of de novo fatty acid synthesis. Associated with immune activation and proliferation.
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Lipid metabolism
Target
Recommended clone
Available fluorescent conjugates
Description
GSS
EPR6563*
Conjugation-ready
Catalyzes glutathione synthesis supporting antioxidant capacity. Associated with immune cell survival.
PRDX2 / PRP
EPR5154
AF® 488,  AF® 647
Peroxide reducing enzyme involved in redox signaling. Associated with immune activation.
SIRT1
E104*
AF® 488,  AF® 647, APC, PE
Peroxide reducing enzyme involved in redox signaling. Associated with immune activation.
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Redox

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Supernova Dye technology is patented by Beckman Coulter, Inc.

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.