JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB176594

Anti-ZZEF1抗体

Anti-ZZEF1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal ZZEF1 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZZEF1 aa 500-600.

查看别名

KIAA0399, ZZEF1, Zinc finger ZZ-type and EF-hand domain-containing protein 1

1 Images
Immunoprecipitation - Anti-ZZEF1 antibody (AB176594)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZZEF1 antibody (AB176594)

Detection of ZZEF1 by Western Blot of Immunprecipitate.
ab176594 at 0.4μg/ml labeling ZZEF1 in 293T whole cell lysate immunoprecipitated using ab176594 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ZZEF1 antibody (ab176594)

Predicted band size: 331 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP

applications

免疫原

Synthetic Peptide within Human ZZEF1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

O43149

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p>ab176594 not performed satisfactorily when used for WB of ZZEF1 in crude preparations (e.g. whole cell lysate), however can be used for WB of enriched (e.g. immunoprecipitated) sources of ZZEF1.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZZEF1 also known as Zinc Finger ZZ-Type with EF-Hand Domain 1 is a protein with a mass of approximately 330 kDa. This protein functions in the cell nucleus and cytoplasm and acts as a transcription factor by binding to DNA and regulating gene expression. It bears zinc finger domains and EF-hand motifs which suggest a role in DNA binding and calcium ion interaction respectively. It's expressed in various tissues including the brain liver and heart highlighting its diverse functional roles in different physiological contexts.
Biological function summary

ZZEF1 influences cell growth and differentiation by participating in transcription regulation. It integrates into multi-protein complexes that modify chromatin structure facilitating changes in gene expression. The protein plays an important part in the transcriptional control of genes responsible for cell cycle progression and apoptosis. This highlights its potential role in balancing cell proliferation and death which is fundamental in development and homeostasis.

Pathways

ZZEF1 is active in the Wnt signaling and Notch signaling pathways both essential for cell fate decisions and tissue development. Within these pathways it can interact with other transcription factors to modulate target gene expression. ZZEF1 has connections to key proteins such as β-catenin in the Wnt pathway and RBPJ in the Notch pathway indicating a collaborative role in gene expression regulation during developmental processes.

ZZEF1 impacts cancer and neurodegenerative diseases through its involvement in transcription and cell cycle regulation. Its altered expression or mutations can drive abnormal cell proliferation linking it to oncogenesis. In certain cancers it interacts with p53 a tumor suppressor protein potentially affecting cell cycle checkpoints. Additionally its dysregulation might contribute to neurodegenerative conditions where cell growth and apoptosis are disturbed suggesting a complex role in maintaining neural tissue integrity.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Histone H3 reader which may act as a transcriptional coactivator for KLF6 and KLF9 transcription factors.
See full target information ZZEF1
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 18:164 PubMed33158444

2020

Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG.

Applications

Unspecified application

Species

Unspecified reactive species

Sofía Espinoza,Sebastián B Arredondo,Francisca Barake,Francisco Carvajal,Fernanda G Guerrero,Fabian Segovia-Miranda,David M Valenzuela,Ursula Wyneken,Alejandro Rojas-Fernández,Waldo Cerpa,Loreto Massardo,Lorena Varela-Nallar,Alfonso González

Nature communications 9:1960 PubMed29773874

2018

Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Shazia Ashraf,Hiroki Kudo,Jia Rao,Atsuo Kikuchi,Eugen Widmeier,Jennifer A Lawson,Weizhen Tan,Tobias Hermle,Jillian K Warejko,Shirlee Shril,Merlin Airik,Tilman Jobst-Schwan,Svjetlana Lovric,Daniela A Braun,Heon Yung Gee,David Schapiro,Amar J Majmundar,Carolin E Sadowski,Werner L Pabst,Ankana Daga,Amelie T van der Ven,Johanna M Schmidt,Boon Chuan Low,Anjali Bansal Gupta,Brajendra K Tripathi,Jenny Wong,Kirk Campbell,Kay Metcalfe,Denny Schanze,Tetsuya Niihori,Hiroshi Kaito,Kandai Nozu,Hiroyasu Tsukaguchi,Ryojiro Tanaka,Kiyoshi Hamahira,Yasuko Kobayashi,Takumi Takizawa,Ryo Funayama,Keiko Nakayama,Yoko Aoki,Naonori Kumagai,Kazumoto Iijima,Henry Fehrenbach,Jameela A Kari,Sherif El Desoky,Sawsan Jalalah,Radovan Bogdanovic,Nataša Stajić,Hildegard Zappel,Assel Rakhmetova,Sharon-Rose Wassmer,Therese Jungraithmayr,Juergen Strehlau,Aravind Selvin Kumar,Arvind Bagga,Neveen A Soliman,Shrikant M Mane,Lewis Kaufman,Douglas R Lowy,Mohamad A Jairajpuri,Richard P Lifton,York Pei,Martin Zenker,Shigeo Kure,Friedhelm Hildebrandt
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB122194

Anti-ZZEF1 antibody

Immunocytochemistry - Anti-ZZEF1 antibody (AB122194)

  • 0
  • 0
Primary Antibodies

AB174652

Anti-ZZEF1 antibody

Immunoprecipitation - Anti-ZZEF1 antibody (AB174652)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB168812

Anti-KIF18B antibody

Western blot - Anti-KIF18B antibody (AB168812)

  • 0
  • 0
Primary Antibodies

AB234744

Anti-YME1L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YME1L1 antibody (AB234744)

  • 0
  • 0
Primary Antibodies

AB74579

Anti-GORASP2/GRASP55 antibody

Western blot - Anti-GORASP2/GRASP55 antibody (AB74579)

  • 0
  • 0
Primary Antibodies

AB157592

Anti-WASH1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-WASH1 antibody - C-terminal (AB157592)

  • 5
  • 1

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com