AB174652

Anti-ZZEF1抗体

Name: ZZEF1抗体 (ab174652)| Abcam中文官网
Brand: Abcam Limited
SKU: AB174652
Price: 4606 CNY
Availability: InStock

Anti-ZZEF1 antibody

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(1 Publication)

Rabbit Polyclonal ZZEF1 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZZEF1 aa 1900-1950.

查看别名

KIAA0399, ZZEF1, Zinc finger ZZ-type and EF-hand domain-containing protein 1

1 Images

Supplier Data

Immunoprecipitation - Anti-ZZEF1 antibody (AB174652)

Detection of Human ZZEF1 by Western Blot of Immunoprecipitates.

Samples : Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.

Lane 1 : ZZEF1 was immunoprecipitated by an other anti-ZZEF1 antibody. Lane 2 : ab174652 was used for IP at 6 μg/mg lysate. Lane 3 : IP with Control IgG. For blotting immunoprecipitated ZZEF1, ab174652 was used at 0.4 μg/ml.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZZEF1 antibody (ab174652)

Predicted band size: 331 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human ZZEF1 aa 1900-1950. The exact immunogen used to generate this antibody is proprietary information.

O43149

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZZEF1 also known as Zinc Finger ZZ-Type with EF-Hand Domain 1 is a protein with a mass of approximately 330 kDa. This protein functions in the cell nucleus and cytoplasm and acts as a transcription factor by binding to DNA and regulating gene expression. It bears zinc finger domains and EF-hand motifs which suggest a role in DNA binding and calcium ion interaction respectively. It's expressed in various tissues including the brain liver and heart highlighting its diverse functional roles in different physiological contexts.

Biological function summary

ZZEF1 influences cell growth and differentiation by participating in transcription regulation. It integrates into multi-protein complexes that modify chromatin structure facilitating changes in gene expression. The protein plays an important part in the transcriptional control of genes responsible for cell cycle progression and apoptosis. This highlights its potential role in balancing cell proliferation and death which is fundamental in development and homeostasis.

Pathways

ZZEF1 is active in the Wnt signaling and Notch signaling pathways both essential for cell fate decisions and tissue development. Within these pathways it can interact with other transcription factors to modulate target gene expression. ZZEF1 has connections to key proteins such as β-catenin in the Wnt pathway and RBPJ in the Notch pathway indicating a collaborative role in gene expression regulation during developmental processes.

ZZEF1 impacts cancer and neurodegenerative diseases through its involvement in transcription and cell cycle regulation. Its altered expression or mutations can drive abnormal cell proliferation linking it to oncogenesis. In certain cancers it interacts with p53 a tumor suppressor protein potentially affecting cell cycle checkpoints. Additionally its dysregulation might contribute to neurodegenerative conditions where cell growth and apoptosis are disturbed suggesting a complex role in maintaining neural tissue integrity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Histone H3 reader which may act as a transcriptional coactivator for KLF6 and KLF9 transcription factors.

See full target information ZZEF1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10350 PubMed39609413

2024

E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Janina A Lehmann,Doris Lindner,Hsu-Min Sung,Georg Stoecklin

View all publications

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Anti-ZZEF1抗体

Anti-ZZEF1 antibody

Immunoprecipitation - Anti-ZZEF1 antibody (AB174652)