Anti-ZWINT抗体(ab71982)
Key features and details
- Rabbit polyclonal to ZWINT
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZWINT抗体
参阅全部 ZWINT 一抗 -
描述
兔多克隆抗体to ZWINT -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a region between residue 225 and the C terminus (residue 277) of human ZWINT (NP_008988.2).
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab71982 was affinity purified using an epitope specific to ZWINT immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71982于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/250.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
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| WB |
1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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ICC/IF
1/50 - 1/250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
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WB
1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 11130 Human
- Omim: 609177 Human
- SwissProt: O95229 Human
- Unigene: 591363 Human
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别名
- Human ZW10 interacting protein 1 antibody
- HZwint 1 antibody
- HZwint1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (ab71982)Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling ZWINT with ab71982 at at 1/200 (1µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
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Western blot - Anti-ZWINT antibody (ab71982)All lanes : Anti-ZWINT antibody (ab71982) at 0.04 µg
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZWINT antibody (ab71982)Detection of ZWINT by Western Blot of Immunprecipitate.
ab71982 at 1µg/ml staining ZWINT in HeLa whole cell lysate immunoprecipitated using ab71982 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab71982 被引用在 3 文献中.
- Wu J et al. A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona. J Cell Biol 223:N/A (2024). PubMed: 37934467
- Mou K et al. Zwint facilitates melanoma progression by promoting c-Myc expression. Exp Ther Med 22:818 (2021). PubMed: 34131441
- Si M et al. Integrated Analysis To Identify Molecular Biomarkers Of High-Grade Serous Ovarian Cancer. Onco Targets Ther 12:10057-10075 (2019). PubMed: 31819501