Anti-ZRSR2抗体(ab223062)
Key features and details
- Rabbit polyclonal to ZRSR2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-ZRSR2抗体 -
描述
兔多克隆抗体to ZRSR2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ZRSR2 aa 150-400.
Database link: Q15696 -
阳性对照
- WB: K562 and HEK-293 wole cell lysates; mouse heart tissue lysate. IHC-P: Human testis tissue. ICC/IF: HepG2 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223062于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
|
|
| WB |
1/500 - 1/5000. Predicted molecular weight: 58 kDa.
|
|
| IHC-P |
1/20 - 1/200.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/200. |
|
WB
1/500 - 1/5000. Predicted molecular weight: 58 kDa. |
|
IHC-P
1/20 - 1/200. |
靶标
-
功能
Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing. -
组织特异性
Widely expressed. -
序列相似性
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain. -
翻译后修饰
Phosphorylated in the RS domain by SRPK1. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 8233 Human
- Entrez Gene: 22184 Mouse
- Omim: 300028 Human
- SwissProt: Q15696 Human
- SwissProt: Q62377 Mouse
- Unigene: 171909 Human
- Unigene: 426178 Mouse
-
别名
- CCCH type zinc finger antibody
- CCCH type zinc finger, RNA binding motif and serine/arginine rich protein 2 antibody
- MGC142014 antibody
see all
图片
-
Western blot - Anti-ZRSR2 antibody (ab223062)All lanes : Anti-ZRSR2 antibody (ab223062) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : mouse heart tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZRSR2 antibody (ab223062)Paraffin embedded human testis tissue stained for ZRSR2 with ab223062 (1/100) in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-ZRSR2 antibody (ab223062)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ZRSR2 (green) using ab223062 (1/100) in ICC/IF. Secondary: Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab223062 尚未被引用在任何文献中。