重组Anti-Zonula occludens蛋白3/ZO3抗体[EPR13414-152] (ab182159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13414-152] to Zonula occludens protein 3/ZO3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Zonula occludens蛋白3/ZO3抗体[EPR13414-152]
参阅全部 Zonula occludens protein 3/ZO3 一抗 -
描述
兔单克隆抗体[EPR13414-152] to Zonula occludens蛋白3/ZO3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Caco-2, MCF-7 cell lysates. Human fetal kidney tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13414-152 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 103 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 103 kDa). |
靶标
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序列相似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
细胞定位
Cell membrane. Cell junction > tight junction. - Information by UniProt
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数据库链接
- Entrez Gene: 27134 Human
- Entrez Gene: 314640 Rat
- Omim: 612689 Human
- SwissProt: O95049 Human
- Unigene: 25527 Human
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别名
- Tight junction protein 3 antibody
- Tight junction protein ZO-3 antibody
- Tight junction protein ZO3 antibody
see all
图片
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All lanes : Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/50000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414-152] (ab182159) at 1/10000 dilution + Human fetal kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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