AB307799

重组Anti-ZO-1抗体[HL1185] - BSA and Azide free

Name: ZO-1抗体[HL1185] (ab307799)| Abcam中文官网
Brand: Abcam Limited
SKU: AB307799
Price: 10123 CNY
Availability: InStock
Rating: 1 (1 reviews)

Anti-ZO-1 antibody [HL1185] - BSA and Azide free

Recombinant
了解详情

(1 Review)

(22 Publications)

Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Dog, Cat, Rat samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP1.

查看别名

ZO1, TJP1, Tight junction protein 1, Tight junction protein ZO-1, Zona occludens protein 1, Zonula occludens protein 1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] - BSA and Azide free (AB307799)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] - BSA and Azide free (AB307799)

ZO-1 antibody [HL1185] (ab307799) detects ZO-1 protein at cell membrane by immunofluorescent analysis in A341 cells fixed in 4% paraformaldehyde at RT for 15 min. ZO-1 stained by ab307799 diluted at 1/500 (green). Fluoroshield with DAPI (blue)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1185

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Dog, Cat, Rat

应用

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human TJP1. The exact immunogen used to generate this antibody is proprietary information.

Q07157

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.

Biological function summary

ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.

Pathways

The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.

ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 7798316, PubMed : 9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed : 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed : 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).

See full target information TJP1

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文献 (22)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

2025

Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

Cell death discovery 11:417 PubMed40883266

2025

METTL3-mediated m6A modification of circCDKAL1 regulates macrophage M1 polarization and nasal epithelial cell barrier function in allergic rhinitis through IGF2BP2/JARID2/HMGB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabin Zhan,Dan Luo,Yunlong Fu,Yu Zhou,Rui Li,Xin Wei

Cellular and molecular life sciences : CMLS 82:262 PubMed40580316

2025

NR4A2 attenuates early brain injury after intracerebral hemorrhage by promoting M2 microglial polarization via TLR4/TRAF6/NF-κB pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Di Hu,Cheng Huang,Ling Tang,Jiawen Lei,Jiaqi Wang,Wenzheng Hu,Minshan Chen,Siyuan Song,Lin Lu,Pingyi Xu

Antioxidants (Basel, Switzerland) 14: PubMed40563324

2025

Dynamic Transcriptomic and Cellular Remodeling Underlie Cuprizone-Induced Demyelination and Endogenous Repair in the CNS.

Applications

Unspecified application

Species

Unspecified reactive species

Yantuanjin Ma,Tianyi Liu,Zhipeng Li,Wei Wei,Qiting Zhao,Shufen Wang

Frontiers in physiology 16:1538733 PubMed40529994

2025

Modulation of gut microbiota and immune response by soy peptides mitigates irinotecan induced intestinal toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfa Jing,Dongli Yan

Diabetology & metabolic syndrome 17:182 PubMed40448159

2025

Gamma -aminobutyric acid ameliorates neurological impairments in type 1 diabetes mellitus mice by regulating the "gut flora-LPS-TLR4-NF-ΚB" signalling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Wang,Lihai Zhang,Xianhe Wang,Jing Dong,Jiaxin Li

BMC medicine 23:285 PubMed40361123

2025

Gut microbiota-derived glutathione from metformin treatment alleviates intestinal ferroptosis induced by ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyan Wang,Xinyu Wang,Chaoyi Wang,Wangxin Yan,Junpeng Xu,Zhengyang Song,Mingli Su,Jingjing Zeng,Qiannian Han,Gaoyi Ruan,Eryao Zhang,Wantie Wang

Glia 73:1565-1588 PubMed40183383

2025

Role of Cx43 and ACKR3 in Modulating Astrocytic Response and Neuronal Survival Post-Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yan,Shenhao Xie,DianDa Chen,Jinlin Xiao,ErMing Zeng,Tao Hong,Jian Duan

Drug design, development and therapy 19:771-791 PubMed39925879

2025

Metagenomic Sequencing Combined with Metabolomics to Explore Gut Microbiota and Metabolic Changes in Mice with Acute Myocardial Infarction and the Potential Mechanism of Allicin.

Applications

Unspecified application

Species

Unspecified reactive species

Yijie Gao,Gaofeng Qin,Shichao Liang,Jiajie Yin,Baofu Wang,Hong Jiang,Mengru Liu,Fangyuan Luo,Xianlun Li

iScience 27:111517 PubMed39759008

2025

The mesenteric adipokine SFRP5 alleviated intestinal epithelial apoptosis improving barrier dysfunction in Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Zhang,Lugen Zuo,Xue Song,Wenjing Zhang,Zi Yang,Zhiyuan Wang,Yibing Guo,Sitang Ge,Lian Wang,Yueyue Wang,Zhijun Geng,Jing Li,Jianguo Hu

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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重组Anti-ZO-1抗体[HL1185] - BSA and Azide free

Anti-ZO-1 antibody [HL1185] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] - BSA and Azide free (AB307799)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (22)

Abcam Product Promise

重组Anti-ZO-1抗体[HL1185] - BSA and Azide free

Anti-ZO-1 antibody [HL1185] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] - BSA and Azide free (AB307799)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

搭配使用产品

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (22)

Abcam Product Promise