Anti-ZNRF1抗体(ab175125)
Key features and details
- Goat polyclonal to ZNRF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ZNRF1抗体 -
描述
山羊多克隆抗体to ZNRF1 -
宿主
Goat -
特异性
ab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1). -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide within Mouse ZNRF1 aa 93-106 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Sequence:SDSTYAHGNGYQET
Database link: Q91V17 -
阳性对照
- Znrf1 transfected N2a cells, Mouse embryo and adult brain lysates and Mouse cerebral cortex tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175125于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 24 kDa.
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 24 kDa. 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to substrates. May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin-protein ligase activity. -
组织特异性
Expressed primarily in the nervous system, with expression higher in developing brain relative to adult. Expressed at low levels in testis and thymus. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger. -
结构域
The RING-type zinc finger domain is required for E3 ligase activity. -
细胞定位
Endosome. Lysosome. Membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Associated with synaptic vesicle membranes in neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 509076 Cow
- Entrez Gene: 84937 Human
- Entrez Gene: 170737 Mouse
- Entrez Gene: 690769 Rat
- Omim: 612060 Human
- SwissProt: F1MM41 Cow
- SwissProt: Q8ND25 Human
- SwissProt: Q91V17 Mouse
see all -
别名
- B830022L21Rik antibody
- DKFZp434E229 antibody
- E3 ubiquitin protein ligase ZNRF1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF1 antibody (ab175125)
Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex, labeling ZNRF1 using ab175125 at 10 µg/ml. Streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.
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Western blot - Anti-ZNRF1 antibody (ab175125)All lanes : Anti-ZNRF1 antibody (ab175125) at 1 µg/ml
Lane 1 : Non-transfected N2a cells
Lane 2 : Mouse Znrf1 transfected N2a cells
Lane 3 : Mouse embryo brain lysate
Lane 4 : Mouse adult brain lysate
Developed using the ECL technique.
Predicted band size: 24 kDaPrimary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab175125 被引用在 1 文献中.
- Hu C et al. circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis. Mol Cancer 21:24 (2022). PubMed: 35045883