Anti-ZNF774抗体(ab122137)
Key features and details
- Rabbit polyclonal to ZNF774
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZNF774抗体 -
描述
兔多克隆抗体to ZNF774 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence:
MWLGTSGKSGLPGHCLENPLQECHPAQLEEWALKGISRPSVISQPEQKEE PWVLPLQNFEARKIPRESHTDC
, corresponding to amino acids 1-72 of Human ZNF774 -
阳性对照
- Human colon tissue; RT4 cell lysate and Human Plasma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122137于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in transcriptional regulation. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 12 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 342132 Human
- SwissProt: Q6NX45 Human
- Unigene: 55307 Human
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别名
- Zinc finger protein 774 antibody
- ZN774_HUMAN antibody
- ZNF774 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ZNF774 antibody (ab122137)Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and cytoskeleton (intermediate filaments). Recommended concentration of ab122137 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF774 antibody (ab122137)ab122137, at 1/200 dilution, staining ZNF774 in Human colon tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. -
Western blot - Anti-ZNF774 antibody (ab122137)All lanes : Anti-ZNF774 antibody (ab122137) at 1/500 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab122137 尚未被引用在任何文献中。