Anti-ZNF316抗体(ab176708)
Key features and details
- Rabbit polyclonal to ZNF316
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZNF316抗体 -
描述
兔多克隆抗体to ZNF316 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human ZNF316 aa 475-525. The exact sequence is proprietary.
Sequence:HQAVHTADRPHCCPDCGQAFRLRADFQRHRRGGGCAEAGGDGPRREPGET A
Database link: A6NFI3 -
阳性对照
- HeLa, 293T and Jurkat whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176708 was affinity purified using an epitope specific to ZNF316 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176708于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 108 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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相关性
May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 15 C2H2-type zinc fingers. Contains 1 KRAB domain. -
细胞定位
Nucleus -
数据库链接
- Entrez Gene: 100131017 Human
- SwissProt: A6NFI3 Human
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别名
- Zinc finger protein 316 antibody
- ZNF316 antibody
图片
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Western blot - Anti-ZNF316 antibody (ab176708)All lanes : Anti-ZNF316 antibody (ab176708) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ZNF316 antibody (ab176708)ab176708 at 0.4 µg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176708 at 6 µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of ZNF316.
Lane 3: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
数据表及文件
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Datasheet download
文献 (0)
ab176708 尚未被引用在任何文献中。
