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重组RabMAb

重组Anti-ZNF217抗体[EPR6362] (ab124927)

Reviews (1) Submit a question
Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
  • OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)
  • Anti-ZNF217 antibody [EPR6362] (ab124927)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6362] to ZNF217
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-ZNF217抗体[EPR6362]
    参阅全部 ZNF217 一抗

  • 描述

    兔单克隆抗体[EPR6362] to ZNF217

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human ZNF217 aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • MCF7, PC3 and Caco2 cell lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124927于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
说明
WB
1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.

  • 序列相似性

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ZABC 1 antibody
      • ZABC1 antibody
      • Zinc finger protein 217 antibody
      • ZN 217 antibody
      • ZN217 antibody
      • ZN217_HUMAN antibody
      • ZNF 217 antibody
      • ZNF217 antibody
      see all

    图片

    • Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
      Western blot - Anti-ZNF217 antibody [EPR6362] (ab124927)
      All lanes : Anti-ZNF217 antibody [EPR6362] (ab124927) at 1/10000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : PC3 cell lysates
      Lane 3 : Caco2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 115 kDa

    • OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)
      OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] (ab124927)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-ZNF217 antibody [EPR6362] (ab124927)
      Anti-ZNF217 antibody [EPR6362] (ab124927)

    文献 (0)

    发表研究结果有使用 ab124927？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124927 尚未被引用在任何文献中。

    客户评价及客户问答

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    Western blot abreview for Anti-ZNF217 antibody [EPR6362]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human ESCs)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    30 µg
    Specification
    human ESCs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 25 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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