Anti-ZNF169抗体(ab225924)
Key features and details
- Rabbit polyclonal to ZNF169
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ZNF169抗体 -
描述
兔多克隆抗体to ZNF169 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ZNF169 aa 1-100.
Database link: Q14929 -
阳性对照
- WB: HepG2 and HeLa whole cell lysate. IHC-P: Human colon cancer tissue and small intestine tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| WB |
1/1000 - 1/5000. Detects a band of approximately 68, 45 kDa (predicted molecular weight: 68 kDa).
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| 说明 |
|---|
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IHC-P
1/20 - 1/200. |
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WB
1/1000 - 1/5000. Detects a band of approximately 68, 45 kDa (predicted molecular weight: 68 kDa). |
靶标
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相关性
ZNF169 belongs to the krueppel C2H2 type zinc finger protein family. It may be involved in transcriptional regulation. It is highly expressed in kidney, weakly expressed in heart, liver, spleen, and small intestine. Not expressed in adult brain or spinal cord. ZNF169 contains thirteen C2H2 type zinc fingers and one KRAB domain. -
细胞定位
Nucleus -
数据库链接
- Entrez Gene: 169841 Human
- Entrez Gene: 67911 Mouse
- Omim: 603404 Human
- SwissProt: Q14929 Human
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别名
- MGC51961 antibody
- Zfp169 antibody
- Zinc finger protein 169 antibody
- Znf169 antibody
图片
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Western blot - Anti-ZNF169 antibody (ab225924)All lanes : Anti-ZNF169 antibody (ab225924) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse kidney tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 45,68 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF169 antibody (ab225924)Paraffin embedded human colon cancer tissue stained for ZNF169 with ab225924 (1/100 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF169 antibody (ab225924)Paraffin embedded human small intestine tissue stained for ZNF169 with ab225924 (1/100 dilution) in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225924 尚未被引用在任何文献中。