AB249878

重组Anti-ZNF160抗体[EPR12523] - BSA and Azide free

Anti-ZNF160 antibody [EPR12523] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ZNF160 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

KIAA1611, ZNF160, Zinc finger protein 160, Zinc finger protein HZF5, Zinc finger protein Kr18, HKr18

3 Images

Flow Cytometry (Intracellular) - Anti-ZNF160 antibody [EPR12523] - BSA and Azide free (AB249878)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZNF160 antibody [EPR12523] - BSA and Azide free (AB249878)

This data was developed using ab175227, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized U87-MG cells labeling ZNF160 with ab175227 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Supplier Data

Immunoprecipitation - Anti-ZNF160 antibody [EPR12523] - BSA and Azide free (AB249878)

This data was developed using ab175227, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from SW480 cell lysate using ab175227.

All lanes:

Immunoprecipitation - Anti-ZNF160 antibody [EPR12523] (<a href='/products/primary-antibodies/znf160-antibody-epr12523-ab175227'>ab175227</a>)

Predicted band size: 94 kDa

false

Supplier Data

Western blot - Anti-ZNF160 antibody [EPR12523] - BSA and Azide free (AB249878)

This data was developed using ab175227, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNF160 antibody [EPR12523] (<a href='/products/primary-antibodies/znf160-antibody-epr12523-ab175227'>ab175227</a>) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Lane 5:

Fetal liver cell lysate at 10 µg

Predicted band size: 94 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ZNF160 antibody [EPR12523]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12523

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249878 is the carrier-free version of ab175227.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZNF160 also known as Zinc Finger Protein 160 is a protein encoded by the ZNF160 gene. It functions as a transcription factor primarily involved in binding to specific DNA sequences to regulate the transcription of genetic information from DNA to mRNA. The molecular mass of ZNF160 is approximately 90 kDa. This protein is predominantly expressed in the nucleus of cells with high expression levels noted in hematopoietic tissues including bone marrow and lymphoid tissues.

Biological function summary

ZNF160 plays an important role in regulating gene expression associated with cellular growth and differentiation. It forms part of multi-protein complexes that exert control over the transcriptional machinery. By modulating the transcription of various genes ZNF160 contributes to the maintenance of normal cellular processes. Its regulatory activities are important in ensuring that cell division and development proceed correctly and it may act as a suppressor or activator depending on the cellular context.

Pathways

ZNF160 engages in pivotal roles within the Wnt signaling pathway and Notch signaling pathway. These pathways are integral to cell fate determination and embryonic development. In the Wnt signaling pathway associations with proteins like β-catenin enable ZNF160 to influence gene expression related to cell cycle progression. Interactions with the Notch signaling pathway involve collaboration with proteins such as Notch1 affecting differentiation processes in various cell types.

ZNF160 has been implicated in certain cancers and developmental disorders. Its dysregulation can lead to aberrant cell proliferation contributing to cancerous growths. ZNF160 in conjunction with proteins associated with cancer progression such as p53 may influence tumor suppression or oncogenic pathways. In developmental disorders mutations or altered expression of ZNF160 lead to defects in cellular differentiation resulting in conditions like congenital malformations. The link between ZNF160 and these disorders highlights the significance of proper regulatory mechanisms in maintaining health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May be involved in transcriptional regulation.

See full target information ZNF160

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-ZNF160抗体[EPR12523] - BSA and Azide free