重组Anti-Zinc Alpha 2 Glycoprotein抗体[EPR14425(B)] (ab180574)
![Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)](https://www.abcam.cn/ps/products/180/ab180574/Images/ab180574-212518-anti-zinc-alpha-2-glycoprotein-antibody-epr14425b-western-blot.jpg "Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Zinc Alpha 2 Glycoprotein抗体[EPR14425(B)]
参阅全部 Zinc Alpha 2 Glycoprotein 一抗 -
描述
兔单克隆抗体[EPR14425(B)] to Zinc Alpha 2 Glycoprotein -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human Plasma and HepG2 cell lines; Human liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14425(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180574于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa).
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| IP |
1/30.
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| IHC-P |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa). |
|
IP
1/30. |
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IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. -
组织特异性
Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 563 Human
- Omim: 194460 Human
- SwissProt: P25311 Human
- Unigene: 546239 Human
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别名
- Alpha 2 glycoprotein 1 zinc antibody
- Alpha 2 glycoprotein 1 zinc binding antibody
- Alpha 2 glycoprotein zinc antibody
see all
图片
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Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg
Secondary
Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)](https://www.abcam.cn/ps/products/180/ab180574/Images/ab180574-212515-anti-zinc-alpha-2-glycoprotein-antibody-epr14425b-western-blot.jpg)
Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-rabbit HRP at 1/1000 dilution
Predicted band size: 34 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)](https://www.abcam.cn/ps/products/180/ab180574/Images/ab180574-212517-anti-zinc-alpha-2-glycoprotein-antibody-epr14425b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.
Blocking/dilution buffer and concentration: 5% NFDM/TBST
All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution
Lane 1 : Human plasma
Lane 2 : Negative control
Secondary
All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)](https://www.abcam.cn/ps/products/180/ab180574/Images/ab180574-212516-anti-zinc-alpha-2-glycoprotein-antibody-epr14425b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)](https://www.abcam.cn/ps/products/180/ab180574/Images/ab180574-5-benefits-of-recombinant-antibodies.png)
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab180574 被引用在 2 文献中.
- Fu X et al. Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. Liver Int 42:696-709 (2022). PubMed: 34854209
- Xue Y et al. Zinc-a-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population. Int J Mol Sci 16:691-703 (2015). WB ; Human . PubMed: 25561225