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AB308399

重组Anti-Zika virus NS4B蛋白抗体[HL1664] - BSA and Azide free

Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free

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Rabbit Recombinant Monoclonal Zika virus NS4B protein antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - Zika virus, Zika virus, Transfected cell lysate - Zika virus samples.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free (AB308399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free (AB308399)

Immunohistochemical analysis of mock and Zika virus NS4B transfected HEK293T labeling Zika virus NS4B protein using ab308399 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free (AB308399)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free (AB308399)

For immunofluorescence analysis, Zika virus non-infected and infected cells slide were permeabilized for detection of Zika virus NS4B protein using ab308399 at 1/100 dilution (green). Blue : Fluoroshield with DAPI.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1664

亚型

IgG

不含载体蛋白

Yes

反应种属

Zika virus

应用

ICC/IF, IHC-P, WB

applications

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搭配使用产品

Primary Antibodies

AB214337

Anti-Flavivirus NS1 antibody [D/2/D6/B7]

Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus NS1 antibody [D/2/D6/B7] (AB214337)

  • 0
  • 0
Primary Antibodies

AB308390

Anti-Dengue virus Type 2 NS5 protein antibody [GT361]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

  • 0
  • 0
Primary Antibodies

AB308183

Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free

Recombinant

Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free (AB308183)

  • 0
  • 0
Primary Antibodies

AB308409

Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Zika virus": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Zika virus": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Zika virus": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Zika virus NS4B sometimes called NS4B nonstructural protein is an integral membrane protein with a mass of approximately 27 kDa. The protein localizes to the endoplasmic reticulum (ER) of infected cells. NS4B functions as an important component in the replication of the viral RNA genome. During viral replication NS4B alters the host cell membrane creating structures that favor viral replication. This protein also plays a role as a suppressor of the host's antiviral immune responses by interfering with the signaling pathways that control cytokines and interferons.
Biological function summary

NS4B interacts closely with other nonstructural proteins to form the viral replication complex a multi-protein assembly essential for efficient RNA synthesis. NS4B is known to modify the lipid composition of the ER membrane facilitating the formation of replication platforms essential for viral RNA synthesis. The protein also modulates cellular stress responses helping the virus to evade host immune surveillance. NS4B factors heavily in viral strategy to manipulate host cell processes ensuring successful viral replication and infection propagation.

Pathways

NS4B influences the interferon signaling pathway and the unfolded protein response. It suppresses interferon production impacting the interferon-stimulated gene (ISG) response by modulating the host's immune signaling cascades. NS4B directly interacts with proteins such as NS4A and NS5 contributing towards the viral RNA polymerase activity. Its interactions stabilize the replication complex enhancing RNA genome synthesis. By inhibiting the unfolded protein response NS4B affects protein homeostasis allowing the virus to continue exploiting host resources.

NS4B's function relates to Zika virus infection and it has associations with microcephaly in newborns. The protein plays a role in the virus’s pathogenic effects especially in neural cells contributing to neurodevelopmental disorders. Additionally NS4B impacts Guillain-Barré syndrome development linking it with immune-mediated damage to peripheral nerves. The interaction of NS4B with other proteins like NS1 helps modulate immune evasion strategies enhancing disease progression and infection severity.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Zika virus NS4B protein
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Abcam Product Promise

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详情请参阅我们的条款与条件。

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