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AB308183

重组Anti-Zika virus NS4B蛋白抗体[HL1663] - BSA and Azide free

Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free

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Rabbit Recombinant Monoclonal Zika virus NS4B protein antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Zika virus, Transfected cell line - Zika virus samples.
2 Images
Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free (AB308183)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free (AB308183)

Immunohistochemical analysis of paraffin-embedded mock and Zika virus NS4B transfected HEK293T labeling Zika virus NS4B protein with ab308183 at 1/1000 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free (AB308183)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free (AB308183)

For immunofluorescence analysis, Zika virus non-infected (left) and infected cells (right) were fixed and permeabilized for detection of Zika virus NS4B protein using ab308183 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1663

亚型

IgG

不含载体蛋白

Yes

反应种属

Zika virus

应用

IHC-P, ICC/IF, WB

applications

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搭配使用产品

Primary Antibodies

AB214337

Anti-Flavivirus NS1 antibody [D/2/D6/B7]

Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus NS1 antibody [D/2/D6/B7] (AB214337)

  • 0
  • 0
Primary Antibodies

AB4648

Anti-GFAP antibody [2A5] - Astrocyte Marker

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] - Astrocyte Marker (AB4648)

  • 4
  • 15
Primary Antibodies

AB308399

Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)

  • 0
  • 0
Primary Antibodies

AB308409

Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Zika virus": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Zika virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Zika virus envelope protein also known as the E protein facilitates the virus's entry into host cells. This protein participates in the virus's attachment and fusion processes. It generally consists of three domains and has an approximate molecular mass of 53 kDa. Expression of this envelope protein occurs on the surface of the Zika virus particle after its synthesis in the host cell's endoplasmic reticulum. E proteins allow the Zika virus to interact with cell receptors aiding in the virus's ability to establish an infection.
Biological function summary

The Zika virus envelope protein initiates fusion with the host cell membrane enabling the release of viral RNA into the host cytoplasm. This process is not solitary; it works as part of a complex involving other viral proteins like the membrane protein (M protein). The E protein has a role in immune system evasion by altering its structure and glycan shielding which complicates recognition by host antibodies. These aspects help the virus persist and replicate within its host.

Pathways

The Zika virus envelope protein engages in the viral entry and replication pathways. A critical pathway involves receptor-mediated endocytosis reliant on host cell receptors to internalize the virus. The E protein works alongside the prM protein a precursor of the M protein to facilitate structural rearrangement necessary for the membrane fusion. The protein also impacts signaling pathways that influence the host's cell cycle which provides a favorable environment for viral replication and expression.

Several are associated with the activity of the Zika virus envelope protein. It is most notably linked to Zika virus infections which can lead to microcephaly and other severe birth defects in newborns when contracted during pregnancy. The envelope protein interacts with host immune proteins like the complement system which sometimes leads to an exaggerated immune response complicating recovery and potentially causing autoimmunity. Zika's E protein shares similarities with envelope proteins of other flaviviruses such as Dengue virus which further complicates immune recognition due to cross-reactive antibodies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Zika virus NS4B protein
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

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