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AB308186

重组Anti-Zika virus Envelope蛋白抗体[HL1699] - BSA and Azide free

Anti-Zika virus Envelope protein antibody [HL1699] - BSA and Azide free

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Rabbit Recombinant Monoclonal Zika virus envelope protein antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Zika virus samples. Immunogen corresponding to Synthetic Peptide within Zika virus Genome polyprotein.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Zika virus Envelope protein antibody [HL1699] - BSA and Azide free (AB308186)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus Envelope protein antibody [HL1699] - BSA and Azide free (AB308186)

For immunofluorescence analysis, Zika virus non-infected (left) and infected cells (right) were fixed and permeabilized for detection of Zika virus Envelope protein using ab308186 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Western blot - Anti-Zika virus Envelope protein antibody [HL1699] - BSA and Azide free (AB308186)
  • WB

Supplier Data

Western blot - Anti-Zika virus Envelope protein antibody [HL1699] - BSA and Azide free (AB308186)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Zika virus Envelope protein antibody [HL1699] - BSA and Azide free (ab308186) at 1/2000 dilution

All lanes:

Zika viral lysate at 0.5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1699

亚型

IgG

不含载体蛋白

Yes

反应种属

Zika virus

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Zika virus Genome polyprotein. The exact immunogen used to generate this antibody is proprietary information.

Q32ZE1

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搭配使用产品

Sample Prep & Detection Kits

AB102921

Streptavidin Conjugation Kit - Lightning-Link®

Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

  • 0
  • 0
Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

  • 0
  • 0
Primary Antibodies

AB214337

Anti-Flavivirus NS1 antibody [D/2/D6/B7]

Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus NS1 antibody [D/2/D6/B7] (AB214337)

  • 0
  • 0
Primary Antibodies

AB308183

Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free

Recombinant

Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] - BSA and Azide free (AB308183)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

KD Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB308399

Anti-Zika virus NS4B protein antibody [HL1664] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Zika virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Zika virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Zika virus envelope protein also known as the E protein facilitates the virus's entry into host cells. This protein participates in the virus's attachment and fusion processes. It generally consists of three domains and has an approximate molecular mass of 53 kDa. Expression of this envelope protein occurs on the surface of the Zika virus particle after its synthesis in the host cell's endoplasmic reticulum. E proteins allow the Zika virus to interact with cell receptors aiding in the virus's ability to establish an infection.
Biological function summary

The Zika virus envelope protein initiates fusion with the host cell membrane enabling the release of viral RNA into the host cytoplasm. This process is not solitary; it works as part of a complex involving other viral proteins like the membrane protein (M protein). The E protein has a role in immune system evasion by altering its structure and glycan shielding which complicates recognition by host antibodies. These aspects help the virus persist and replicate within its host.

Pathways

The Zika virus envelope protein engages in the viral entry and replication pathways. A critical pathway involves receptor-mediated endocytosis reliant on host cell receptors to internalize the virus. The E protein works alongside the prM protein a precursor of the M protein to facilitate structural rearrangement necessary for the membrane fusion. The protein also impacts signaling pathways that influence the host's cell cycle which provides a favorable environment for viral replication and expression.

Several are associated with the activity of the Zika virus envelope protein. It is most notably linked to Zika virus infections which can lead to microcephaly and other severe birth defects in newborns when contracted during pregnancy. The envelope protein interacts with host immune proteins like the complement system which sometimes leads to an exaggerated immune response complicating recovery and potentially causing autoimmunity. Zika's E protein shares similarities with envelope proteins of other flaviviruses such as Dengue virus which further complicates immune recognition due to cross-reactive antibodies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Zika virus envelope protein
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