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Tags & Cell Markers Cell Type Markers Epi / Endo-thelial

抗斑马鱼Gut Secretory Cell Epitopes抗体[FIS 2F11/2] (ab71286)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (35)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)

    Key features and details

    • Mouse monoclonal [FIS 2F11/2] to Zebrafish Gut Secretory Cell Epitopes
    • Suitable for: IHC-P
    • Reacts with: Zebrafish
    • Isotype: IgG1

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    概述

    • 产品名称

      抗斑马鱼Gut Secretory Cell Epitopes抗体[FIS 2F11/2]
    • 描述

      小鼠单克隆抗体[FIS 2F11/2] to斑马鱼Gut Secretory Cell Epitopes
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Zebrafish
    • 免疫原

      Tissue, cells or virus corresponding to Zebrafish Zebrafish Gut Secretory Cell Epitopes. Tissue/cell preparation: Lysate of zebrafish intestine.

    • 阳性对照

      • IHC: Zebrafish Larvae (5 days)
    • 常规说明

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.02% Sodium azide
      Constituent: 99.98% PBS
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 克隆

      单克隆
    • 克隆编号

      FIS 2F11/2
    • 骨髓瘤

      Sp2/0-Ag14
    • 同种型

      IgG1
    • 轻链类型

      kappa
    • 研究领域

      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial
      • Signal Transduction
      • Growth Factors/Hormones
      • Hormones
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Differentiation
      • Stem Cells
      • Signaling Pathways
      • Notch
      • Surface Molecules
      • Developmental Biology
      • Organogenesis
      • Gut development
      • Gut specification

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab71286于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 0.5 µg/ml.
    说明
    IHC-P
    Use a concentration of 0.5 µg/ml.

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)

      IHC image of Zebrafish Gut Secretory Cell Epitopes staining in a section of formalin-fixed paraffin-embedded Zebra fish Larvae (5 days) performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab71286, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (35)

    发表研究结果有使用 ab71286?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab71286 被引用在 35 文献中.

    • Karolczak S  et al. Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy. J Clin Invest 133:N/A (2023). PubMed: 37490339
    • Song J  et al. The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway. NPJ Regen Med 8:20 (2023). PubMed: 37024481
    • Ali RQ  et al. Loss of zebrafish pkd1l1 causes biliary defects that have implications for biliary atresia splenic malformation. Dis Model Mech 16:N/A (2023). PubMed: 37675454
    • Yang D  et al. Roxadustat induces hepatotoxicity in zebrafish embryos via inhibiting Notch signaling. J Appl Toxicol 43:1073-1082 (2023). PubMed: 36755374
    • Zhao C  et al. Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signaling. Proc Natl Acad Sci U S A 119:e2201097119 (2022). PubMed: 36469766
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    Could you provide the Zebrafish IHC-whole mount protocol?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 28 2016

    Answer

    This antibody has cited to be used in whole mount immunohistochemistry in a number of publications e.g. Dev Biol 376:187-97 (2013), Dev Biol 372:178-89 (2012),   Development 138:1339-48 (2011), Development 138:3557-68 (2011). The protocol researchers have used is cited in http://www.nature.com/ng/journal/v39/n3/full/ng1961.html (Dong et. al.) so we recommend reading this article for protocol. We do not test this antibody in our laboratory so in case it doesn't show expected results please try the possible troubleshooting. In case of any question please do not hesitate to contact  Abcam Scientific Support Team.

    Read More

    Padamjeet Singh

    Abcam Scientific Support

    回复于 Jan 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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