Anti-ZDHHC9抗体(ab74504)
Key features and details
- Rabbit polyclonal to ZDHHC9
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZDHHC9抗体
参阅全部 ZDHHC9 一抗 -
描述
兔多克隆抗体to ZDHHC9 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide derived from C-terminal region of human ZDHHC9.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74504于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
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IHC-P
1/50 - 1/100. |
靶标
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功能
The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. -
组织特异性
Highly expressed in kidney, skeletal muscle, brain, lung and liver. Absent in thymus, spleen and leukocytes. -
疾病相关
Defects in ZDHHC9 are the cause of mental retardation syndromic X-linked ZDHHC9-related (MRXSZ) [MIM:300799]. A disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Some patients have marfanoid habitus as an additional feature. -
序列相似性
Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
Contains 1 DHHC-type zinc finger. -
结构域
The DHHC domain is required for palmitoyltransferase activity. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 51114 Human
- Entrez Gene: 208884 Mouse
- Entrez Gene: 302808 Rat
- Omim: 300646 Human
- SwissProt: Q9Y397 Human
- SwissProt: P59268 Mouse
- Unigene: 193566 Human
- Unigene: 207367 Mouse
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别名
- Antigen MMSA 1 antibody
- Asp His His Cys domain containing protein 9 antibody
- CGI89 antibody
see all
图片
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Western blot - Anti-ZDHHC9 antibody (ab74504)All lanes : Anti-ZDHHC9 antibody (ab74504) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 26 kDa, 34 kDa, 48 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC9 antibody (ab74504)Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74504. Left: untreated sample. Right: sample treated with immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab74504 被引用在 3 文献中.
- Chong X et al. ZDHHC9 promotes colon tumor growth by inhibiting effector T cells. Oncol Lett 25:5 (2023). PubMed: 36419754
- Yao H et al. A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chem Biol 2:192-205 (2021). PubMed: 34458782
- Cambronne XA et al. Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach. Proc Natl Acad Sci U S A 109:20473-8 (2012). WB . PubMed: 23184980