Anti-ZDHHC13抗体(ab28759)
Key features and details
- Goat polyclonal to ZDHHC13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZDHHC13抗体 -
描述
山羊多克隆抗体to ZDHHC13 -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms (as represented by NP_061901 and NP_001001483). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide:
(C)FHPAREKVLRSV
, corresponding to C terminal amino acids 611-622 of Human ZDHHC13 Peptide is available as ab45682 - ZDHHC13 peptide (611-622) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28759于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
应用说明
WB: Use at a concentration of 0.1 - 3 µg/ml.
This antibody gave a positive result in ELISA against the immunizing peptide.
Antibody detection limit dilution 1:32,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody gave a positive result in ELISA against the immunizing peptide.
Antibody detection limit dilution 1:32,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Palmitoyltransferase for HD and GAD2 (By similarity). Mediates Mg(2+) transport. -
序列相似性
Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.
Contains 6 ANK repeats.
Contains 1 DHHC-type zinc finger. -
结构域
The DHHC domain is required for palmitoyltransferase activity. -
细胞定位
Golgi apparatus membrane. Cytoplasmic vesicle membrane. Low extracellular Mg(2+) induces increase in Golgi and in post-Golgi vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 54503 Human
- Entrez Gene: 243983 Mouse
- Entrez Gene: 365252 Rat
- Omim: 612815 Human
- SwissProt: Q8IUH4 Human
- SwissProt: Q9CWU2 Mouse
- Unigene: 188569 Human
- Unigene: 279116 Mouse
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别名
- DHHC 13 antibody
- DHHC-13 antibody
- FLJ10852 antibody
see all
图片
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Western blot - Anti-ZDHHC13 antibody (ab28759)Anti-ZDHHC13 antibody (ab28759) at 0.1 µg/ml + Human cerebellum lysate at 35 µg
Developed using the ECL technique.Primary antibody was incubated for 1 hour.
数据表及文件
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Datasheet download
文献 (3)
ab28759 被引用在 3 文献中.
- Sun Y et al. AMPK Phosphorylates ZDHHC13 to Increase MC1R Activity and Suppress Melanomagenesis. Cancer Res 83:1062-1073 (2023). PubMed: 36701140
- Chen S et al. Targeting MC1R depalmitoylation to prevent melanomagenesis in redheads. Nat Commun 10:877 (2019). PubMed: 30787281
- Chen S et al. Palmitoylation-dependent activation of MC1R prevents melanomagenesis. Nature 549:399-403 (2017). WB . PubMed: 28869973
