Anti-ZCWCC1抗体(ab14429)
Key features and details
- Rabbit polyclonal to ZCWCC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZCWCC1抗体
参阅全部 ZCWCC1 一抗 -
描述
兔多克隆抗体to ZCWCC1 -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human ZCWCC1 aa 600-700. The epitope recognised by ab14429 maps to a region of human zinc finger, CW-type with coiled-coil domain 1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified using an epitope specific to ZCWCC1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14429于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 3 µg/ml.
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| WB |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 118 kDa.
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| 说明 |
|---|
|
IP
Use a concentration of 3 µg/ml. |
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WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 118 kDa. |
靶标
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功能
Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation. May act as a transcriptional repressor. Down-regulates CA9 expression. -
组织特异性
Highly expressed in smooth muscle, pancreas and testis. -
序列相似性
Contains 1 CW-type zinc finger. -
细胞定位
Nucleus. Cytoplasm, cytosol. Mainly located in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 22880 Human
- SwissProt: Q9Y6X9 Human
- Unigene: 555918 Human
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别名
- AC004542.C22.1. antibody
- CW type with coiled coil domain 1 antibody
- KIAA0852 antibody
see all
图片
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Western blot - Anti-ZCWCC1 antibody (ab14429)All lanes : Anti-ZCWCC1 antibody (ab14429) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 118 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZCWCC1 antibody (ab14429)Immunoprecipitation of ZCWCC1 with ab14429 at 3 µg/mL.
Lane 1: ab14429 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab14429 被引用在 2 文献中.
- Zhang J et al. Microrchidia family CW-type zinc finger 2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N-myc downstream regulated gene 1 promoter. Int J Mol Med 49:N/A (2022). PubMed: 34913078
- Guddeti RK et al. The chromatin modifier MORC2 affects glucose metabolism by regulating the expression of lactate dehydrogenase A through a feed forward loop with c-Myc. FEBS Lett 595:1289-1302 (2021). PubMed: 33626175