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Anti-ZCCHC6抗体(ab76901)

Submit a review Q&A (2)
Western blot - Anti-ZCCHC6 antibody (ab76901)
  • Western blot - Anti-ZCCHC6 antibody (ab76901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (ab76901)

Key features and details

  • Mouse polyclonal to ZCCHC6
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-ZCCHC6抗体
    参阅全部 ZCCHC6 一抗

  • 描述

    小鼠多克隆抗体to ZCCHC6

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).

  • 阳性对照

    • Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76901于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 171, 48 kDa.

Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.
IHC-P
1/50.
说明
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 171, 48 kDa.

Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.
IHC-P
1/50.

靶标

  • 相关性

    ZCCHC6 contains 3 CCHC-type zinc fingers.

  • 数据库链接

    • 别名

      • KIAA1711 antibody
      • MGC38585 antibody
      • PAP associated domain containing 6 antibody
      • PAPD6 antibody
      • Zinc finger CCHC domain containing 6 antibody
      see all

    图片

    • Western blot - Anti-ZCCHC6 antibody (ab76901)
      Western blot - Anti-ZCCHC6 antibody (ab76901)
      Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 171, 48 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-ZCCHC6 antibody (ab76901)
      Western blot - Anti-ZCCHC6 antibody (ab76901)
      All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml

      Lane 1 : ZCCHC6 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 171, 48 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?



      Note: predicted molecular weight for isoform 5 is 48 kDa.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (ab76901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (ab76901)

      ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection. 

    文献 (0)

    发表研究结果有使用 ab76901？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76901 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Our customer had test following your suggestion and I have transfer the file. Check this please.  

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    Answer

    Thank you for your message and for providing this further information. I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement or a credit note in compensation. Please note that, unfortunately, we cannot offer an alternative catalog reference or another lot of ab76901. If your customer wants a replacement it will be from the same lot. I look forward to hearing from you with details of how you would like to proceed.

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    Question

    LOT NUMBER GR44511-2 ORDER NUMBER ********** DESCRIPTION OF THE PROBLEM Non-specific band SAMPLE Hela cell, whole cell lysate PRIMARY ANTIBODY Concentration or dilution : 1:100, 1:500 Diluent buffer : 1% Milk in PBS-T Incubation time : 2hr & Overnight Incubation temperature: 2hr at RT, Overnight at 4℃ Wash Buffer : PBST (0.1% Tween20) Number of washes 3 times DETECTION METHOD Chemidoc(Biorad) POSITIVE AND NEGATIVE CONTROLS USED - ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION Lysis buffer : 200mM KCl, 20mM Tris, 0.2mM EDTA, 0.1% Triton-X100 Protease inhibitors: : Yes(Sigma PI cocktail) Phosphatase inhibitors : No Reducing agent : No Boiling for ≥5 min? yes/no : Yes AMOUNT OF PROTEIN LOADED 50ug/lane ELECTROPHORESIS/GEL CONDITIONS Fig1. 10% Fig2. 8% TRANSFER AND BLOCKING CONDITIONS Type of membrane : PVDF Protein transfer verified : WB same blot with another antibody  Yes Blocking agent and concentration : 5% Milk in PBS-T Blocking time : 1hr, Blocking temperature : RT(20℃) SECONDARY ANTIBODY Pierce 31430 Goat anti-mouse IgG(H+L) Concentration or dilution : 1:5000 Diluent buffer : PBST Incubation time : 40min, 1hr Incubation temperature: RT Fluorochrome or enzyme conjugate: HRP Wash Buffer : PBST (0.1% Tween20) Number of washes 3 times HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Primary concentration, incubation time, incubation temperature ADDITIONAL NOTES -

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    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results: When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : Click here for the western blot image using ab9385 (or use the following: www.abcam.com/ab9385). Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement or a credit note. I hope this information is helpful, and I thank you for your cooperation.

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