AB76901

Anti-ZCCHC6抗体

Name: ZCCHC6抗体 (ab76901)| Abcam中文官网
Brand: Abcam Limited
SKU: AB76901
Price: 4606 CNY
Availability: InStock

Anti-ZCCHC6 antibody

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(1 Publication)

Mouse Polyclonal ZCCHC6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human TUT7.

查看别名

HS2, KIAA1711, ZCCHC6, TUT7, Terminal uridylyltransferase 7, TUTase 7, Zinc finger CCHC domain-containing protein 6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (AB76901)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (AB76901)

ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection.

Unknown

Western blot - Anti-ZCCHC6 antibody (AB76901)

All lanes:

Western blot - Anti-ZCCHC6 antibody (ab76901) at 5 µg/mL

All lanes:

human liver tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 171 kDa

Observed band size: 150 kDa

false

Unknown

Western blot - Anti-ZCCHC6 antibody (AB76901)

Note : predicted molecular weight for isoform 5 is 48 kDa.

All lanes:

Western blot - Anti-ZCCHC6 antibody (ab76901) at 5 µg/mL

Lane 1:

ZCCHC6 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 171 kDa

Observed band size: 53 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Native Full Length Protein corresponding to Human TUT7.

Q5VYS8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZCCHC6 also known as Zinc finger CCHC-type containing 6 is a protein that binds RNA and has a mass of about 66 kDa. It belongs to the terminal uridylyl transferase (TUTases) family and functions by adding uridines to the 3' end of RNA substrates. This protein shows expression in multiple tissues including the brain testes and ovaries reflecting its potential involvement in diverse cellular processes.

Biological function summary

ZCCHC6 contributes to RNA metabolism influencing RNA stability translation and degradation. It participates in the post-transcriptional regulation of gene expression by adding uridines to mRNA and non-coding RNAs which can impact the decay and processing of these RNA molecules. ZCCHC6 is also part of a dynamic complex that includes components like poly(A) polymerases interacting with various cofactors to modulate RNA length and stability.

Pathways

The processes involving ZCCHC6 play significant roles in RNA processing pathways and in the regulation of gene expression. Its activity contributes to the RNA surveillance pathway which ensures the quality control of RNA transcripts. ZCCHC6 interacts with other key proteins such as ZCCHC11 which shares similar protein domains and functions in RNA uridylation reinforcing its cooperation in these pathways.

ZCCHC6 influences conditions linked to RNA dysregulation such as certain cancers and neurodegenerative diseases. The protein plays a role in maintaining RNA quality and abundance and disturbances in its function can lead to abnormal RNA profiles observed in these disorders. In cancer for example aberrant activity of ZCCHC6 might disrupt normal cell regulatory mechanisms potentially interacting with proteins like LIN28 to promote oncogenic processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed : 19703396, PubMed : 25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed : 25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed : 25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed : 22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed : 25979828, PubMed : 28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed : 25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed : 18172165, PubMed : 19703396, PubMed : 22898984, PubMed : 25480299, PubMed : 25979828, PubMed : 28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed : 30122351).

See full target information TUT7

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 148: PubMed34128984

2021

HoxD transcription factors define monosynaptic sensory-motor specificity in the developing spinal cord.

Applications

Unspecified application

Species

Unspecified reactive species

Fumiyasu Imai,Mike Adam,S Steven Potter,Yutaka Yoshida

View all publications

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Anti-ZCCHC6抗体

Anti-ZCCHC6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (AB76901)

Western blot - Anti-ZCCHC6 antibody (AB76901)

Western blot - Anti-ZCCHC6 antibody (AB76901)