AB226307

Anti-ZCCHC6抗体

Name: ZCCHC6抗体 (ab226307)| Abcam中文官网
Brand: Abcam Limited
SKU: AB226307
Price: 4748 CNY
Availability: InStock

Anti-ZCCHC6 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal ZCCHC6 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TUT7 aa 1400 to C-terminus.

查看别名

HS2, KIAA1711, ZCCHC6, TUT7, Terminal uridylyltransferase 7, TUTase 7, Zinc finger CCHC domain-containing protein 6

1 Images

Supplier Data

Immunoprecipitation - Anti-ZCCHC6 antibody (AB226307)

ZCCHC6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226307 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226307 at 1 μg/ml.

Lane 1 : ab226307 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ZCCHC6 antibody (ab226307)

Predicted band size: 171 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human TUT7 aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5VYS8

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Giant Panda": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab226307 was affinity purified using an epitope specific to ZCCHC6 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ZCCHC6 also known as Zinc finger CCHC-type containing 6 is a protein that binds RNA and has a mass of about 66 kDa. It belongs to the terminal uridylyl transferase (TUTases) family and functions by adding uridines to the 3' end of RNA substrates. This protein shows expression in multiple tissues including the brain testes and ovaries reflecting its potential involvement in diverse cellular processes.

Biological function summary

ZCCHC6 contributes to RNA metabolism influencing RNA stability translation and degradation. It participates in the post-transcriptional regulation of gene expression by adding uridines to mRNA and non-coding RNAs which can impact the decay and processing of these RNA molecules. ZCCHC6 is also part of a dynamic complex that includes components like poly(A) polymerases interacting with various cofactors to modulate RNA length and stability.

Pathways

The processes involving ZCCHC6 play significant roles in RNA processing pathways and in the regulation of gene expression. Its activity contributes to the RNA surveillance pathway which ensures the quality control of RNA transcripts. ZCCHC6 interacts with other key proteins such as ZCCHC11 which shares similar protein domains and functions in RNA uridylation reinforcing its cooperation in these pathways.

ZCCHC6 influences conditions linked to RNA dysregulation such as certain cancers and neurodegenerative diseases. The protein plays a role in maintaining RNA quality and abundance and disturbances in its function can lead to abnormal RNA profiles observed in these disorders. In cancer for example aberrant activity of ZCCHC6 might disrupt normal cell regulatory mechanisms potentially interacting with proteins like LIN28 to promote oncogenic processes.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed : 19703396, PubMed : 25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed : 25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed : 25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed : 22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed : 25979828, PubMed : 28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed : 25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed : 18172165, PubMed : 19703396, PubMed : 22898984, PubMed : 25480299, PubMed : 25979828, PubMed : 28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed : 30122351).

See full target information TUT7

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 14:926904 PubMed35978952

2022

Intraneuronal sortilin aggregation relative to granulovacuolar degeneration, tau pathogenesis and sorfra plaque formation in human hippocampal formation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Jiang,Chen Yang,Jia-Qi Ai,Qi-Lei Zhang,Xiao-Lu Cai,Tian Tu,Lily Wan,Xiao-Sheng Wang,Hui Wang,Aihua Pan,Jim Manavis,Wei-Ping Gai,Chong Che,Ewen Tu,Xiao-Ping Wang,Zhen-Yan Li,Xiao-Xin Yan

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZCCHC6抗体

Anti-ZCCHC6 antibody

Immunoprecipitation - Anti-ZCCHC6 antibody (AB226307)