Anti-ZBTB48抗体- N-terminal (ab225935)
Key features and details
- Rabbit polyclonal to ZBTB48 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ZBTB48抗体- N-terminal -
描述
兔多克隆抗体to ZBTB48 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Orangutan
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免疫原
Recombinant fragment corresponding to Human ZBTB48 aa 1-300 (N terminal).
Database link: P10074 -
阳性对照
- WB: HEK-293T, K562, HepG2 and U-251 MG whole cell lysates; Mouse liver tissue lysate. IHC-P: Human spleen and lung tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225935于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
|
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| WB |
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
|
| 说明 |
|---|
|
IHC-P
1/20 - 1/200. |
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WB
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
靶标
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功能
Binds to and regulates the J and/or S elements in MHC II promoter. -
组织特异性
Detected in adrenal gland and neuroblastoma. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 11 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3104 Human
- Entrez Gene: 100090 Mouse
- Omim: 165270 Human
- SwissProt: P10074 Human
- SwissProt: Q1H9T6 Mouse
- Unigene: 502330 Human
- Unigene: 24661 Mouse
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别名
- 0610011D15Rik antibody
- AI327031 antibody
- GLI Kruppel family member HKR3 antibody
see all
图片
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Western blot - Anti-ZBTB48 antibody - N-terminal (ab225935)All lanes : Anti-ZBTB48 antibody - N-terminal (ab225935) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5 : Mouse liver tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB48 antibody - N-terminal (ab225935)Paraffin-embedded human spleen tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB48 antibody - N-terminal (ab225935)Paraffin-embedded human lung tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225935 尚未被引用在任何文献中。