Anti-ZAP70 (phospho Y315)抗体(ab60970)
Key features and details
- Rabbit polyclonal to ZAP70 (phospho Y315)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZAP70 (phospho Y315)抗体
参阅全部 ZAP70 一抗 -
描述
兔多克隆抗体to ZAP70 (phospho Y315) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human ZAP70 (phospho Y315).
Database link: P43403 -
阳性对照
- Lymph node tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60970于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
|
|
| IHC-P | (1) |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. -
组织特异性
Expressed in T- and natural killer cells. -
疾病相关
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains. -
结构域
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. -
翻译后修饰
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. -
细胞定位
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 7535 Human
- Entrez Gene: 22637 Mouse
- Omim: 176947 Human
- SwissProt: P43403 Human
- SwissProt: P43404 Mouse
- Unigene: 234569 Human
- Unigene: 8038 Mouse
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别名
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 (phospho Y315) antibody (ab60970)ab60970 at 1/50 - 1/100 dilution staining ZAP70 in human lymph node by immunohistochemistry on paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
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Western blot - Anti-ZAP70 (phospho Y315) antibody (ab60970)All lanes : Anti-ZAP70 (phospho Y315) antibody (ab60970) at 500 cells
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) treated with 40 nM Ca2+, whole cell lysate
Lane 2 : Jurkat incubated with 40 nM Ca2+, whole cell lysate incubated with phospho-peptide
Predicted band size: 69 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60970 尚未被引用在任何文献中。