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Anti-ZAP70抗体[ZAP-03] (ab38871)

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Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
  • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
  • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
  • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
  • Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)
  • Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)

Key features and details

  • Mouse monoclonal [ZAP-03] to ZAP70
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-ZAP70抗体[ZAP-03]
    参阅全部 ZAP70 一抗

  • 描述

    小鼠单克隆抗体[ZAP-03] to ZAP70

  • 宿主

    Mouse

  • 特异性

    ab38871 recognises ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment representing C-terminal part (160 amino acids) of human ZAP-70.

  • 阳性对照

    • WB: HPB-ALL human peripheral blood leukemia T-cell line. Flow Cyt (Intra): Human B-CLL peripheral blood.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab38871于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 2 - 5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (1)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
说明
Flow Cyt (Intra)
Use a concentration of 2 - 5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

靶标

  • 功能

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

  • 组织特异性

    Expressed in T- and natural killer cells.

  • 疾病相关

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.

  • 结构域

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

  • 翻译后修饰

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

  • 细胞定位

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC 2.7.10.2 antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain of T cell receptor associated protein kinase 70 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)

      Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using ab38871 (concentration in sample 9 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).

    • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)

      Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using ab38871 (concentration in sample 9 μg/ml, GAM APC).

    • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)

      Flow cytometry intracellular staining pattern of human peripheral whole blood using ab38871 (concentration in sample 9 μg/ml, GAM APC).

    • Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (ab38871)

      Intracellular Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations: T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red). 

       

    • Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Lane 1 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/ml
      Lane 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/ml
      Lane 3 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/ml
      Lane 4 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
      Lane 5 : ZAP-70 specific antibody 1E7.2 at 1 µg/ml
      Lane 6 : Isotypic control PPV-06 (mouse IgG1).

      All lanes : HPB-ALL peripheral blood leukemia T-cell line

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 70 kDa
      Additional bands at: 55 kDa (possible IgG), 55 kDa (possible degradation product)



      Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel; Reducing conditions. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
    • Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)
      Lanes 1-2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
      Lanes 3-4 : Isotypic control PPV-06 (mouse IgG1)

      Lanes 1 & 3 : HPB-ALL cell line
      Lanes 2 & 4 : RAMOS cell line

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 70 kDa



      Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.

    文献 (0)

    发表研究结果有使用 ab38871？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab38871 尚未被引用在任何文献中。

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    Western blot abreview for Anti-ZAP70 antibody [ZAP-03]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (gastric cancer)
    Specification
    gastric cancer
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Feb 17 2014

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