Anti-ZAK抗体(ab65249)
Key features and details
- Rabbit polyclonal to ZAK
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZAK抗体
参阅全部 ZAK 一抗 -
描述
兔多克隆抗体to ZAK -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide derived from C terminus of human ZAK
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阳性对照
- HeLa cell extracts and HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| WB |
1/500 - 1/1000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
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| 说明 |
|---|
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ICC/IF
1/500 - 1/1000. |
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WB
1/500 - 1/1000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). |
靶标
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功能
Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'. -
组织特异性
Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation. - Information by UniProt
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数据库链接
- Entrez Gene: 51776 Human
- Entrez Gene: 65964 Mouse
- Omim: 609479 Human
- SwissProt: Q9NYL2 Human
- SwissProt: Q9ESL4 Mouse
- Unigene: 444451 Human
- Unigene: 304143 Mouse
- Unigene: 314618 Mouse
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别名
- AZK antibody
- Cervical cancer suppressor gene 4 protein antibody
- HCCS 4 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ZAK antibody (ab65249)ab65249 at 1/500 dilution staining ZAK in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Western blot - Anti-ZAK antibody (ab65249)All lanes : Anti-ZAK antibody (ab65249) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab65249 尚未被引用在任何文献中。