重组Anti-Ym-1 + Ym-2抗体[EPR15263] (ab192029)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
-
产品名称
Anti-Ym-1 + Ym-2抗体[EPR15263]
参阅全部 Ym-1 + Ym-2 一抗 -
描述
兔单克隆抗体[EPR15263] to Ym-1 + Ym-2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt (Intra) -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Mouse lung, mouse brain, mouse heart, C6, Raw264.7, PC-12 and NIH3T3 lysates. IHC-P: Mouse liver tissue. ICC/IF: Raw264.7 cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15263 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192029于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 45 kDa (predicted molecular weight: 44, 45 kDa).
|
|
IHC-P | (2) |
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
ICC/IF |
1/200.
|
说明 |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 45 kDa (predicted molecular weight: 44, 45 kDa). |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/200. |
靶标
-
相关性
Chitinase 3 like protein 3: Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy. Chitinase 3 like protein 4: Has low chemotactic activity for eosinophils. May play a role in inflammation and allergy. Has no chitinase activity. -
细胞定位
Chitinase 3 like protein 3: Secreted. Rough endoplasmic reticulum lumen. Nucleus envelope. Cytoplasm. Cytoplasmic granule. Note: Predominantly localizes to the lumen of rough endoplasmic reticulum (rER) and nuclear envelope in alveolar macrophages. Localizes to the dilated lumen of rER in immature neutrophils in spleen and in cytoplasmic granules in peritoneal neutrophils. Detected in needle-shaped crystals present in the cytoplasm of bone marrow macrophages. Chitinase 3 like protein 4: Secreted. Cytoplasm. Note: Detected in the cytoplasm of keratinocytes. -
数据库链接
- Entrez Gene: 104183 Mouse
- Entrez Gene: 12655 Mouse
- SwissProt: O35744 Mouse
- SwissProt: Q91Z98 Mouse
-
别名
- Chi3l3 antibody
- Beta-N-acetylhexosaminidase Ym1 antibody
- Chi3l4 antibody
see all
图片
-
Immunofluorescent analysis of paraformaldehyde-fixed Raw264.7 cells labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/200, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 (ab150077) and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution; 2. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution.
-
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/500 with prediluted HRP polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
All lanes : Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/10000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : C6 lysate
Lane 4 : Raw264.7 lysate
Lane 5 : PC-12 lysate
Lane 6 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44, 45 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/50000 dilution + Mouse lung lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44, 45 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (21)
ab192029 被引用在 21 文献中.
- Wang X et al. MicroRNA-382 Promotes M2-Like Macrophage via the SIRP-α/STAT3 Signaling Pathway in Aristolochic Acid-Induced Renal Fibrosis. Front Immunol 13:864984 (2022). PubMed: 35585990
- Kurotani R et al. Development of Aging-Related Emphysematous and Lymphoma-Like Lesions is Enhanced by the Lack of Secretoglobin 3A2 in Mouse Lungs. Int J Chron Obstruct Pulmon Dis 17:1247-1260 (2022). PubMed: 35651829
- He S et al. Sphingomyelin synthase 2 is a positive regulator of the CSF1R-STAT3 pathway in pancreatic cancer-associated macrophage. Front Pharmacol 13:902016 (2022). PubMed: 36324684
- Bouredji Z et al. Testing the efficacy of a human full-length OPG-Fc analog in a severe model of cardiotoxin-induced skeletal muscle injury and repair. Mol Ther Methods Clin Dev 21:559-573 (2021). PubMed: 33997104
- Pi XJ et al. Saponins from Panax japonicus attenuate cognitive impairment in ageing rats through regulating microglial polarisation and autophagy. Pharm Biol 59:1117-1125 (2021). PubMed: 34403300