重组Anti-Yes1抗体[EPR3172] (ab133314)
![Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)](https://www.abcam.cn/ps/products/133/ab133314/Images/ab133314-146807-anti-yes1-antibody-epr3172-western-blot.jpg "Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3172] to Yes1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Yes1抗体[EPR3172]
参阅全部 Yes1 一抗 -
描述
兔单克隆抗体[EPR3172] to Yes1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Yes1 aa 1-100 (internal sequence). The exact sequence is proprietary.
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阳性对照
- U87-MG, Molt-4, LnCaP and A431 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3172 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
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数据库链接
- Entrez Gene: 7525 Human
- Omim: 164880 Human
- SwissProt: P07947 Human
- Unigene: 194148 Human
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形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
图片
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Western blot - Anti-Yes1 antibody [EPR3172] (ab133314)All lanes : Anti-Yes1 antibody [EPR3172] (ab133314) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
![Anti-Yes1 antibody [EPR3172] (ab133314)](https://www.abcam.cn/ps/products/133/ab133314/Images/ab133314-2-benefits-of-recombinant-antibodies.png)
Anti-Yes1 antibody [EPR3172] (ab133314)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133314 被引用在 1 文献中.
- Goya Grocin A et al. Whole Proteome Profiling of N-Myristoyltransferase Activity and Inhibition Using Sortase A. Mol Cell Proteomics 18:115-126 (2019). PubMed: 30341083