AB133314

重组Anti-Yes1抗体[EPR3172]

Anti-Yes1 antibody [EPR3172]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Yes1 antibody. Suitable for WB, IHC-Fr and reacts with Human samples. Cited in 1 publication.

查看别名

YES, YES1, Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes

4 Images

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] (AB133314)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] (AB133314)

Immunohistochemical analysis of Yes1 staining in a section of frozen human normal lung performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab133314, 1/1000 dilution (1µg/ml), for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] (AB133314)

Immunohistochemical analysis of Yes1 staining in a section of frozen human placenta performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab133314, 1/1000 dilution (1µg/ml), for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Yes1 antibody [EPR3172] (AB133314)

Immunohistochemical analysis of Yes1 staining in a section of frozen human frozen breast cancer performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab133314, 1/1000 dilution (1µg/ml), for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

Unknown

Western blot - Anti-Yes1 antibody [EPR3172] (AB133314)

All lanes:

Western blot - Anti-Yes1 antibody [EPR3172] (ab133314) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 10 µg

Lane 2:

Molt-4 cell lysate at 10 µg

Lane 3:

LnCaP cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Yes1 antibody [EPR3172] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3172

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Yes1 protein also known as p61-Yes is a member of the Src family kinases (SFKs) and has a molecular mass of approximately 61 kDa. This non-receptor tyrosine kinase engages in phosphorylation of tyrosine residues playing a significant role in cell signaling. It is widely expressed in many tissues including the brain heart and lungs suggesting its involvement in varied physiological processes.

Biological function summary

The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.

Pathways

The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.

Research connects Yes1 protein to several types of cancer such as colon cancer and breast cancer. The dysregulation of Yes1 along with associated proteins like c-Myc may lead to uncontrolled cell proliferation typical of tumorigenesis. Understanding these interactions offers insights into potential therapeutic targets as altering Yes1 activity might influence cancer progression or treatment outcomes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed : 26979622).

See full target information YES1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 18:115-126 PubMed30341083

2018

Whole Proteome Profiling of -Myristoyltransferase Activity and Inhibition Using Sortase A.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Goya Grocin,Remigiusz A Serwa,Julia Morales Sanfrutos,Markus Ritzefeld,Edward W Tate

View all publications

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重组Anti-Yes1抗体[EPR3172]