Anti-Yes1抗体(ab109744)
Key features and details
- Rabbit polyclonal to Yes1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Yes1抗体
参阅全部 Yes1 一抗 -
描述
兔多克隆抗体to Yes1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Zebrafish
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免疫原
Recombinant Human Yes1 protein.
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阳性对照
- A431 and NIH 3T3 whole cell lysates; Human breast and placenta tissues; HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109744于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
|
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| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
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数据库链接
- Entrez Gene: 507632 Cow
- Entrez Gene: 403917 Dog
- Entrez Gene: 7525 Human
- Entrez Gene: 22612 Mouse
- Entrez Gene: 24884 Rat
- Entrez Gene: 407620 Zebrafish
- Omim: 164880 Human
- SwissProt: Q28923 Dog
see all -
形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
图片
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Western blot - Anti-Yes1 antibody (ab109744)Anti-Yes1 antibody (ab109744) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Western blot - Anti-Yes1 antibody (ab109744)Anti-Yes1 antibody (ab109744) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody (ab109744)ab109744, at 10 µg/ml, staining Yes1 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Yes1 antibody (ab109744)ab109744, at 10 µg/ml, staining Yes1 in formalin fixed, paraffin embedded Human placenta tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-Yes1 antibody (ab109744)Immunofluorescence analysis of Yes1 in methanol fixed HeLa cell, using ab109744 at a 1/200 dilution.
The upper image was stained with Hoesch 33342
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109744 被引用在 3 文献中.
- Ou W et al. Circ_0081143 Contributes to Gastric Cancer Malignant Development and Doxorubicin Resistance by Elevating the Expression of YES1 by Targeting mziR-129-2-3p. Gut Liver 16:861-874 (2022). PubMed: 35686503
- Zhu H et al. Matrine Regulates Proliferation, Apoptosis, Cell Cycle, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Through the circFUT8/miR-944/YES1 Axis. Cancer Manag Res 13:3429-3442 (2021). PubMed: 33907466
- Li R et al. miR-125a-5p promotes gastric cancer growth and invasion by regulating the Hippo pathway. J Clin Lab Anal 35:e24078 (2021). PubMed: 34708891