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重组RabMAb

重组Anti-YAP1 (phospho S127)抗体[EP1675Y] - BSA and Azide free (ab172374)

Submit a review Submit a question References (3)
Dot Blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
  • Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127) - BSA and Azide free
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-YAP1 (phospho S127)抗体[EP1675Y] - BSA and Azide free
    参阅全部 YAP1 一抗

  • 描述

    兔单克隆抗体[EP1675Y] to YAP1 (phospho S127) - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Stimulation may be required to allow detection of the phosphorylated protein.  Please see images below for recommended treatment conditions and positive controls.

  • 经测试应用

    适用于: WB, IHC-P, Dot blotmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab172374 is the carrier-free version of ab76252.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab172374于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65-75 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65-75 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot
Use at an assay dependent concentration.

靶标

  • 功能

    Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

  • 组织特异性

    Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

  • 序列相似性

    Belongs to the YORKIE family.
    Contains 2 WW domains.

  • 翻译后修饰

    Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.

  • 细胞定位

    Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 65 kDa Yes associated protein antibody
    • 65 kDa Yes-associated protein antibody
    • COB1 antibody
    • YAp 1 antibody
    • YAP 65 antibody
    • YAP antibody
    • YAP-1 antibody
    • YAP1 antibody
    • YAP1_HUMAN antibody
    • YAP2 antibody
    • YAP65 antibody
    • yes -associated protein delta antibody
    • Yes associated protein 1 65kDa antibody
    • Yes associated protein 1 antibody
    • Yes associated protein 2 antibody
    • yes associated protein beta antibody
    • YKI antibody
    • Yorkie homolog antibody
    see all

图片

  • Dot Blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
    Dot Blot - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

    Dot blot analysis of YAP1 (pS127) peptide (Lane 1) and YAP1 non-phospho peptide (Lane 2) labelling YAP1 (pS127) with ab76252 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of endometrium tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).

  • Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)
    Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free (ab172374)

文献 (3)

发表研究结果有使用 ab172374？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab172374 被引用在 3 文献中.

  • Zhao L  et al. lncRNA MALAT1 modulates cancer stem cell properties of liver cancer cells by regulating YAP1 expression via miR-375 sponging. Mol Med Rep 22:1449-1457 (2020). PubMed: 32626943
  • Zhang C  et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). PubMed: 30039887
  • Zhang C  et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). PubMed: 29416763

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