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重组RabMAb

重组Anti-XRN2抗体[EPR28598-13] - BSA and Azide free (ab318133)

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Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Immunocytochemistry/ Immunofluorescence - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Flow Cytometry (Intracellular) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
  • Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28598-13] to XRN2 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-XRN2抗体[EPR28598-13] - BSA and Azide free
    参阅全部 XRN2 一抗

  • 描述

    兔单克隆抗体[EPR28598-13] to XRN2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HeLa transfected with siRNA specifically targeting XRN2, LN-229, NIH/3T3, Mouse lung, Mouse skeletal muscle, Mouse brain, Rat skeletal muscle, Rat brain, Human cerebellum and Human liver lysates. IHC-P: Human lung, Human cerebrum, Mouse lung, Mouse cerebrum and Rat cerebrum tissues. ICC/IF: HeLa cells. Flow Cyt(Intra): HeLa cells.

  • 常规说明

    ab318133 is the carrier-free version of ab318132.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318133于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 相关性

    XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

  • 细胞定位

    Nucleus, nucleolus.

  • 数据库链接

    • 别名

      • 5' 3' exoribonuclease 2 antibody
      • DHM1 like protein antibody
      • DHP protein antibody
      • XRN 2 antibody

    图片

    • Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      All lanes : Anti-XRN2 antibody [EPR28598-13] (ab318132) at 1/1000 dilution

      Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : HeLa transfected with siRNA specifically targeti XRN2 whole cell lysate
      Lane 3 : LN-229 (human brain glioblastoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 108 kDa why is the actual band size different from the predicted?


      Exposure time: 8 seconds


      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      All lanes : Anti-XRN2 antibody [EPR28598-13] (ab318132) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 2 : Mouse lung tissue lysate
      Lane 3 : Mouse skeletal muscle tissue lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : Rat skeletal muscle tissue lysate
      Lane 6 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 108 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The identity of the lower MW band at approximately 55 kDa is unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Western blot - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      All lanes : Anti-XRN2 antibody [EPR28598-13] (ab318132) at 1/1000 dilution

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : Human liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/5000 dilution

      Observed band size: 108 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling XRN2 with ab318132 at 1/1000 (0.501 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in human lung.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling XRN2 with ab318132 at 1/1000 (0.501 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in human cerebrum.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling XRN2 with ab318132 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in mouse lung.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling XRN2 with ab318132 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in mouse cerebrum.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling XRN2 with ab318132 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in rat cerebrum.
      The section was incubated with ab318132 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Immunocytochemistry/ Immunofluorescence - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling XRN2 with ab318132 at 1/100 (5.01 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing nuclear staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Flow Cytometry (Intracellular) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Flow Cytometry (Intracellular) - Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

      This data was developed using ab318132, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling XRN2 with ab318132 at 1/500 dilution (0.1 ug)/Magenta (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)
      Anti-XRN2 antibody [EPR28598-13] - BSA and Azide free (ab318133)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab318133 尚未被引用在任何文献中。

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