重组Anti-XRCC4抗体[EPR28958-63] (ab317697)
![Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-541985-anti-xrcc4-antibody-epr28958-63-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-transfected-with-scrambled-sirna-control-whole-cell-lysate-20-ug.jpg "Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28958-63] to XRCC4
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-XRCC4抗体[EPR28958-63]
参阅全部 XRCC4 一抗 -
描述
兔单克隆抗体[EPR28958-63] to XRCC4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WBmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HeLa transfected with siRNA specifically targeting XRCC4 whole cell lysate, Untreated HeLa, HeLa treated with 2uM staurosporine for 3h whole cell lysate, HCT 116, 293T cells, T-47D and Human tonsil lysates. IHC-P: Human tonsil, Human endometrioid carcinoma and Human breast tissues. IP: HeLa cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28958-63 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IP |
1/1000.
|
|
| WB |
1/1000. Predicted molecular weight: 38 kDa.
|
| 说明 |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/1000. |
|
WB
1/1000. Predicted molecular weight: 38 kDa. |
靶标
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功能
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the XRCC4 family. -
翻译后修饰
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7518 Human
- Omim: 194363 Human
- SwissProt: Q13426 Human
- Unigene: 567359 Human
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别名
- DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA repair protein XRCC4 antibody
- SSMED antibody
see all
图片
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Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)All lanes : Anti-XRCC4 antibody [EPR28958-63] (ab317697) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting XRCC4 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 35,55 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
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![Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-541986-anti-xrcc4-antibody-epr28958-63-western-blot-untreated-hela-human-cervical-adenocarcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)All lanes : Anti-XRCC4 antibody [EPR28958-63] (ab317697) at 1/1000 dilution
Lane 1 : Untreated heLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 2uM staurosporine for 3h whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 35,55 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of caspase cleaved XRCC4 is upregulated upon induction of apoptosis (PMID: 33725486).
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
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![Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-541987-anti-xrcc4-antibody-epr28958-63-western-blot-hct-116-human-colorectal-carcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)All lanes : Anti-XRCC4 antibody [EPR28958-63] (ab317697) at 1/1000 dilution
Lanes 1 & 6 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lanes 2 & 5 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lanes 3 & 7 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Lane 1-Lane 4, lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
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![Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-541988-anti-xrcc4-antibody-epr28958-63-western-blot-human-tonsil-tissue-lysate-20-ug.jpg)
Western blot - Anti-XRCC4 antibody [EPR28958-63] (ab317697)Anti-XRCC4 antibody [EPR28958-63] (ab317697) at 1/1000 dilution + Human tonsil tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-2-anti-xrcc4-antibody-epr28958-63-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] (ab317697)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling XRCC4 with ab317697 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human tonsil.
The section was incubated with ab317697 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-3-anti-xrcc4-antibody-epr28958-63-immunohistochemistry-human-endometrioid-c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] (ab317697)Immunohistochemical analysis of paraffin-embedded Human endometrioid carcinoma tissue labeling XRCC4 with ab317697 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human endometrioid carcinoma.
The section was incubated with ab317697 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-4-anti-xrcc4-antibody-epr28958-63-immunohistochemistry-human-breast.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC4 antibody [EPR28958-63] (ab317697)Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling XRCC4 with ab317697 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human breast.
The section was incubated with ab317697 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunoprecipitation - Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-5-anti-xrcc4-antibody-epr28958-63-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-XRCC4 antibody [EPR28958-63] (ab317697)XRCC4 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317697 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317697 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab317697 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317697 in HeLa whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds.
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![Anti-XRCC4 antibody [EPR28958-63] (ab317697)](https://www.abcam.cn/ps/products/317/ab317697/Images/ab317697-1-BenefitsofRecombinantantibodies.jpg)
Anti-XRCC4 antibody [EPR28958-63] (ab317697)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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