Anti-XRCC3抗体(ab97390)
Key features and details
- Rabbit polyclonal to XRCC3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-XRCC3抗体
参阅全部 XRCC3 一抗 -
描述
兔多克隆抗体to XRCC3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 202 and 346 of XRCC3
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阳性对照
- HepG2 and HeLa cell lines
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97390于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. -
序列相似性
Belongs to the RecA family. RAD51 subfamily. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair. - Information by UniProt
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数据库链接
- Entrez Gene: 7517 Human
- Omim: 600675 Human
- SwissProt: O43542 Human
- Unigene: 592325 Human
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别名
- CMM6 antibody
- DNA repair protein XRCC3 antibody
- RAD51 like antibody
see all
图片
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Western blot - Anti-XRCC3 antibody (ab97390)Anti-XRCC3 antibody (ab97390) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 38 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-XRCC3 antibody (ab97390)ab97390, at a 1/200 dilution, staining XRCC3 in paraformaldehyde fixed HeLa by Immunofluorescence.
Lower image was merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97390 被引用在 1 文献中.
- Prensner JR et al. PCAT-1, a long noncoding RNA, regulates BRCA2 and controls homologous recombination in cancer. Cancer Res 74:1651-60 (2014). WB . PubMed: 24473064