Anti-XRCC3抗体(ab58467)
Key features and details
- Rabbit polyclonal to XRCC3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-XRCC3抗体
参阅全部 XRCC3 一抗 -
描述
兔多克隆抗体to XRCC3 -
宿主
Rabbit -
特异性
ab58467 detects endogenous levels of total XRCC1 protein. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Mouse
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免疫原
Synthetic peptide derived from internal sequence of human XRCC3.
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阳性对照
- Extracts from HepG2 cells; human lung carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 38 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
1/100 - 1/500. |
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WB
1/500 - 1/1000. Predicted molecular weight: 38 kDa. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. -
序列相似性
Belongs to the RecA family. RAD51 subfamily. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair. - Information by UniProt
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数据库链接
- Entrez Gene: 7517 Human
- Entrez Gene: 74335 Mouse
- Omim: 600675 Human
- SwissProt: O43542 Human
- SwissProt: Q9CXE6 Mouse
- Unigene: 592325 Human
- Unigene: 19082 Mouse
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别名
- CMM6 antibody
- DNA repair protein XRCC3 antibody
- RAD51 like antibody
see all
图片
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Western blot - Anti-XRCC3 antibody (ab58467)All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution
Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide
Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide
Predicted band size: 38 kDa
Observed band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody (ab58467)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated. -
Immunocytochemistry/ Immunofluorescence - Anti-XRCC3 antibody (ab58467)ab58467 at 1/500 dilution staining XRCC3 in COS7 cells by Immunofluorescence, in the absence or presence of the synthesized peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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