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重组RabMAb

重组Anti-XRCC1抗体[EPR4389(2)] - BSA and Azide free (ab226150)

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Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
  • Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
  • Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4389(2)] to XRCC1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-XRCC1抗体[EPR4389(2)] - BSA and Azide free
    参阅全部 XRCC1 一抗

  • 描述

    兔单克隆抗体[EPR4389(2)] to XRCC1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.

  • 常规说明

    ab226150 is the carrier-free version of ab134056.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226150于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

  • 序列相似性

    Contains 2 BRCT domains.

  • 翻译后修饰

    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.

  • 细胞定位

    Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

图片

  • Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    This data was developed using ab134056, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lanes 1-2: 3 minutes.

    Lanes 3-4: 20 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)

    This data was developed using ab134056, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.


  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)

    This data was developed using ab134056, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
  • Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Western blot - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A375 cell lysate
    Lane 3 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 69 kDa



    This data was developed using ab134056, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    This data was developed using ab134056, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Immunocytochemistry/ Immunofluorescence - Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    This data was developed using ab134056, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.
  • Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)
    Anti-XRCC1 antibody [EPR4389(2)] - BSA and Azide free (ab226150)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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