Anti-XPNPEP3抗体(ab224211)
Key features and details
- Rabbit polyclonal to XPNPEP3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-XPNPEP3抗体 -
描述
兔多克隆抗体to XPNPEP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Orangutan
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免疫原
Recombinant fragment corresponding to Human XPNPEP3 aa 50-250.
Database link: Q9NQH7 -
阳性对照
- IHC-P: Human stomach tissue. WB: NIH/3T3, NBT-II, PC-12, RT4, EFO-21 and A431 cell lysate. Human liver tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224211于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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组织特异性
Isoform 1 and isoform 2 are widely expressed, with isoform 1 being more abundant. -
疾病相关
Defects in XPNPEP3 are the cause of nephronophthisis-like nephropathy type 1 (NPHPL1) [MIM:613159]. A disorder with features of nephronophthisis, a cystic kidney disease leading to end-stage renal failure. Nephronophthisis is histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Typical clinical manifestation are chronic renal failure, anemia, polyuria, polydipsia, isosthenuria, and growth retardation. Associations with extrarenal symptoms are frequent. In NPHPL1 patients, extrarenal symptoms include hypertension, essential tremor, sensorineural hearing loss and gout. Severely affected individuals can manifest a mitochondrial disorder with isolated complex I deficiency activity in muscle, seizures, mental retardation and hypertrophic dilated cardiomyopathy. -
序列相似性
Belongs to the peptidase M24B family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 63929 Human
- Entrez Gene: 321003 Mouse
- Entrez Gene: 685823 Rat
- Omim: 613553 Human
- SwissProt: Q9NQH7 Human
- SwissProt: B7ZMP1 Mouse
- SwissProt: Q5R9W8 Orangutan
- SwissProt: B5DEQ3 Rat
see all -
别名
- Aminopeptidase P3 antibody
- APP3 antibody
- NPHPL1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP3 antibody (ab224211)
Paraffin embedded human stomach tissue stained for XPNPEP3 with ab224211 (1/200 dilution) in immunohistochemical analysis.
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Western blot - Anti-XPNPEP3 antibody (ab224211)All lanes : Anti-XPNPEP3 antibody (ab224211) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 57 kDa -
Western blot - Anti-XPNPEP3 antibody (ab224211)All lanes : Anti-XPNPEP3 antibody (ab224211) at 1/250 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Predicted band size: 57 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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