Anti-XPNPEP1抗体[OTI1E3] (ab123929)
![Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.cn/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)")
Key features and details
- Mouse monoclonal [OTI1E3] to XPNPEP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-XPNPEP1抗体[OTI1E3]
参阅全部 XPNPEP1 一抗 -
描述
小鼠单克隆抗体[OTI1E3] to XPNPEP1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human XPNPEP1. Produced in HEK293T cells (NP_065116).
Database link: Q9NQW7 -
阳性对照
- WB: HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 whole cell lysate. IHC-P: Human thyroid and prostate tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab123929 is purified from Mouse ascites fluid by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI1E3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123929于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 70 kDa.
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| IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| 说明 |
|---|
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WB
1/2000. Predicted molecular weight: 70 kDa. |
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IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
靶标
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功能
Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. -
组织特异性
Expressed in all tissues tested, including pancreas, heart, muscle, kidney, liver, lung and brain. Highest levels in pancreas. -
序列相似性
Belongs to the peptidase M24B family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 486880 Dog
- Entrez Gene: 7511 Human
- Entrez Gene: 170750 Mouse
- Entrez Gene: 170751 Rat
- Omim: 602443 Human
- SwissProt: Q9NQW7 Human
- SwissProt: Q6P1B1 Mouse
- SwissProt: O54975 Rat
see all -
形式
X-prolyl aminopeptidase is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. There are 2 isoforms produced by alternative splicing. -
别名
- Aminoacylproline aminopeptidase antibody
- aminopeptidase P, cytosolic antibody
- APP1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY XPNPEP1, stained for XPNPEP1 (Green) using ab123929 at 1/100 dilution in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.cn/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)Paraffin-embedded human thyroid tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.cn/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)Paraffin-embedded human prostate tissue stained for XPNPEP1 using ab123929 at 1/150 dilution in immunohistochemical analysis.
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![Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.cn/ps/products/123/ab123929/Images/XPNPEP1-Primary-antibodies-ab123929-5.jpg)
Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY XPNPEP1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 70 kDa -
![Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)](https://www.abcam.cn/ps/products/123/ab123929/Images/ab123929-255898-pic1.jpg)
Western blot - Anti-XPNPEP1 antibody [OTI1E3] (ab123929)All lanes : Anti-XPNPEP1 antibody [OTI1E3] (ab123929) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SVT2
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 70 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab123929 被引用在 2 文献中.
- Rao SD et al. M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome. Nat Chem Biol 18:565-574 (2022). PubMed: 35165443
- Drendel V et al. Proteome profiling of clear cell renal cell carcinoma in von Hippel-Lindau patients highlights upregulation of Xaa-Pro aminopeptidase-1, an anti-proliferative and anti-migratory exoprotease. Oncotarget 8:100066-100078 (2017). PubMed: 29245961