Anti-XPG抗体[8H7] (ab46)

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. I am sorry to confirm that as far as we are aware this antibody has not been tested in ICC-IF and regrettably usually we would not be able to provide our 6 month guarantee for this application. All tested applications and species covered by the guarantee are listed on the individual datasheets. I'm sorry the attachment of the publication you found did not come through and I would appreciate if you are able to try sending a this again so we have evidence that this clone has previously worked in this application.

2. Could you confirm how long was the fixation and permeabilisation step? I can suggest this should be 10 minutes only.

3. Could you confirm if you are fixing before or after the staining with the antibody? I can suggest to fix before antibody staining.

4. I can suggest trying the antibody on its own without the PCNA antibody double stain. It is often more effective to optimize antibodies individually before using them in multiple staining.

5. I can recommend to try overnight incubation at 4oC with the primary antibody. This can often provide more efficient and specific staining.

6. Try eliminating glycine from the buffers.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I fully understand your concerns and I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch.

I would also like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and IP and in human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would much appreciate if you are able to provide the following.

1. I am sorry we do not have access to all publications. I would appreciate if you are able to provide an attached copy of the publication you mention. It will be helpful for me to review the details in this.

2. I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


Order Details
Antibody code: ab46

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

Thank you for your patience. The antibody was used in the following publications which I hope will be of use to you: Evans E et al. 1997. EMBO J. 16:625-638. Constantinou A et al. 1999. J. Biol. Chem. 274:5637-5648. Araujo SJ et al. 2001. Mol. Cell. Biol. 21(7):2281-91. At this time this is all the information that I have. What type(s) of cell lystae did you use? At what dilution did you use ab46 at?

The MW of the the human XPG protein is 185 kDa and clone 8H7 binds between human XPG residues Ser 947 and Ala 1165. If you have any more questions, please contact us again.

I am still waiting for details regarding ab46 and will contact you when I receive them. For ab6264, the originator has informed me that the epitope has not been determined.

This antibody has not been tested on paraffin sections.

This antibody has not yet been tested in mouse.