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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair

Anti-XPG抗体[8H7] (ab46)

  • Datasheet
Reviews (1)Q&A (7)References (3)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [8H7] to XPG
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-XPG抗体[8H7]
    参阅全部 XPG 一抗
  • 描述

    小鼠单克隆抗体[8H7] to XPG
  • 宿主

    Mouse
  • 经测试应用

    适用于: IP, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human XPG.
    Database link: P28715

  • 表位

    8H7 binds between human XPG residues Ser 947 and Ala 1165.
  • 常规说明

    Works well on crude cell extract.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    8H7
  • 骨髓瘤

    Sp2
  • 同种型

    IgG2a
  • 轻链类型

    unknown
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab46于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP (1)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
  • 疾病相关

    Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
  • 序列相似性

    Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P28715 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2073 Human
    • Omim: 133530 Human
    • SwissProt: P28715 Human
    • Unigene: 258429 Human
    • 别名

      • COFS 3 antibody
      • COFS3 antibody
      • DNA excision repair protein ERCC 5 antibody
      • DNA excision repair protein ERCC-5 antibody
      • DNA excision repair protein ERCC5 antibody
      • DNA repair protein complementing XP G cells antibody
      • DNA repair protein complementing XP-G cells antibody
      • DNA repair protein complementing XPG cells antibody
      • ERCC 5 antibody
      • ERCC5 antibody
      • ERCC5_HUMAN antibody
      • ERCM 2 antibody
      • ERCM2 antibody
      • Excision repair cross complementation group 5 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency antibody
      • Excision repair cross complementing rodent repair deficiency complementation group 5 antibody
      • Excision repair protein antibody
      • OTTHUMP00000064902 antibody
      • UVDR antibody
      • Xeroderma Pigmentosum Complementation Group G antibody
      • Xeroderma pigmentosum complementation group G protein antibody
      • Xeroderma pigmentosum group G complementing protein antibody
      • Xeroderma pigmentosum group G-complementing protein antibody
      • XPG antibody
      • XPG complementing protein antibody
      • XPGC antibody
      see all

    实验方案

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab46?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab46 被引用在 3 文献中.

    • Ðuraševic S  et al. The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats. Int J Mol Sci 20:N/A (2019). PubMed: 31731785
    • Trego KS  et al. The DNA repair endonuclease XPG interacts directly and functionally with the WRN helicase defective in Werner syndrome. Cell Cycle 10:1998-2007 (2011). Human . PubMed: 21558802
    • Igoucheva O  et al. Involvement of ERCC1/XPF and XPG in oligodeoxynucleotide-directed gene modification. Oligonucleotides 16:94-104 (2006). WB ; Human . PubMed: 16584298

    客户评价及客户问答

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    1-8 of 8 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-XPG antibody [8H7]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cell)
    Total protein in input
    700 µg
    Specification
    HeLa cell
    Immuno-precipitation step
    Protein A/G
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Roberta Guglielmino

    Verified customer

    提交于 Apr 11 2007

    Question

    Inquiry: Your e-mail adress technical@abcam.com is not working. So, here is the e-mail I wanted to send you.

    Thank you for your prompt reply. You will find: 1. the answers to your questions 2. the pdf of the paper I was talking about 3. a powerpoint with photos of my experiment.
    Thank you very much for your attention and I am looking forward hearing from you. As we are going to order this week an antibody for another protein, is it possible to send us at the same time few microliters of 2-3 other lots of XPG antibody to test.
    Thank you.
    Best regards,


    Order Details
    Antibody code: ab46
    Lot number: GR20849-2
    Purchase order number ######
    or preferably Abcam order number:

    General Information Antibody storage conditions (temperature/reconstitution etc) -20oC

    Description of the problem (high background, low signal, non-specific satining etc.) non-specific local staining after local ultraviolet irradiation


    Sample (Species/Tissue/Cell Type/Cell Line etc.) the MRC5 cell line

    Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.) 2% paraformaldehyde

    Antigen retrieval (Enzymatic method, Heat mediated technique etc.)

    Permeabilization step 0.1% triton X100

    Blocking conditions (Buffer/time period, Blocking agent etc.) PBS+0.15%glycine+0.5%BSA, 1h

    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) anti-XPG ab46 (abcam, mouse, dilution 1/500 in PBS+0.15%glycine+0.5%BSA)+ anti-PCNA ab18197 (abcam, rabbit, dilution 1/1000 in PBS+0.15%glycine+0.5%BSA) 2 hours, 3x 5 minutes wash step: PBS + 0.1% triton X100

    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) AlexaFluor 488 goat anti-mouse (invitrogen, goat, dilution 1/1000 in PBS+0.15%glycine+0.5%BSA)+ AlexaFluor 594 goat anti-rabbit (invitrogen, goat, dilution 1/1000 in PBS+0.15%glycine+0.5%BSA) 1 hour, 3x 5 minutes wash step: PBS + 0.1% triton X100, PBS, water before mounting with DAPI.

    Detection method Zeiss microscope

    Positive and negative controls used (please specify) We expect a co-localization with PCNA.

    Optimization attempts (problem solving) dilutions 1/200, 1/500, 1/1000 +/- CSK100 before fixation with PAF 2% permeabilization with Methanol or Triton X-100

    How many times have you tried the IHC? 4


    Have you run a No Primary control? Yes No


    Do you obtain the same results every time? Yes No What steps have you altered? Additional

    Notes We would appreciate if you are also able to provide and image which woudl help us to assess the results

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2012

    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

    1. I am sorry to confirm that as far as we are aware this antibody has not been tested in ICC-IF and regrettably usually we would not be able to provide our 6 month guarantee for this application. All tested applications and species covered by the guarantee are listed on the individual datasheets. I'm sorry the attachment of the publication you found did not come through and I would appreciate if you are able to try sending a this again so we have evidence that this clone has previously worked in this application.

    2. Could you confirm how long was the fixation and permeabilisation step? I can suggest this should be 10 minutes only.

    3. Could you confirm if you are fixing before or after the staining with the antibody? I can suggest to fix before antibody staining.

    4. I can suggest trying the antibody on its own without the PCNA antibody double stain. It is often more effective to optimize antibodies individually before using them in multiple staining.

    5. I can recommend to try overnight incubation at 4oC with the primary antibody. This can often provide more efficient and specific staining.

    6. Try eliminating glycine from the buffers.

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Abcam Scientific Support

    回复于 Oct 12 2012

    Question

    Inquiry: Good afternoon, We bought last June your antibody . Unfortunately, it did not work. I used exactly the same protocole (same cells, same UV-dose, same fixation...) than the one described in the paper: "Generation of DNA single-strand displacement by compromised nucleotide excision repair." (Godon C, Mourgues S, Nonnekens J, Mourcet A, Coin F, Vermeulen W, Mari PO, Giglia-Mari G. EMBO J. 2012 Aug 331(17):3550-63. doi: 10.1038/emboj.2012.193.PMID:22863773,where they used an antibody anti-XPG clone 8H7, like yours, but from Upstate. As you can see in the slide attached to this e-mail, whereas I was expecting to see with your antibody a local staining (a colocalization with the protein PCNA), I observed a staining of the whole nucleus with your antibody. I also tryed many dilutions, + or - CSK100 before fixation of the cells with PAF4%, methanol versus triton X100 permeabilization... I never observed a local staining of XPG whereas in many other papers using the same clone 8H7 but from other companies, they did. Could you please check if you had a problem with the lot I received: GR20849-2. Thank you very much for your understanding and have a nice week.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 09 2012

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I fully understand your concerns and I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch.

    I would also like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and IP and in human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would much appreciate if you are able to provide the following.

    1. I am sorry we do not have access to all publications. I would appreciate if you are able to provide an attached copy of the publication you mention. It will be helpful for me to review the details in this.

    2. I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    I would appreciate if you could also provide an image which would help us to assess the results.

    Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


    Order Details
    Antibody code: ab46

    Lot number:

    Purchase order number
    or preferably Abcam order number:


    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, low signal, non-specific satining etc.)

    Sample (Species/Tissue/Cell Type/Cell Line etc.)


    Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


    Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


    Permeabilization step


    Blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Detection method


    Positive and negative controls used (please specify)


    Optimization attempts (problem solving)
    How many times have you tried the IHC?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No


    What steps have you altered?


    Additional Notes


    We would appreciate if you are also able to provide and image which woudl help us to assess the results

    Read More

    Abcam Scientific Support

    回复于 Oct 09 2012

    Question

    Thanks for the information. We used XPG antibody to detect the levels of XPG protein by Western blotting. We used 100 ug cell lysate and see only a non-specific band size 110 kDa and no any band sized 185 kDa. It will be nice of you if you give us technical tips to get the specific 185 kDa band. Any paper published where people used this antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 25 2004

    Answer

    Thank you for your patience. The antibody was used in the following publications which I hope will be of use to you: Evans E et al. 1997. EMBO J. 16:625-638. Constantinou A et al. 1999. J. Biol. Chem. 274:5637-5648. Araujo SJ et al. 2001. Mol. Cell. Biol. 21(7):2281-91. At this time this is all the information that I have. What type(s) of cell lystae did you use? At what dilution did you use ab46 at?

    Read More

    Abcam Scientific Support

    回复于 Jul 01 2004

    Question

    Ab46 - what size band is detected on a Western? Has the epitope been determined? Ab6264 - Has the epitope been determined?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2004

    Answer

    The MW of the the human XPG protein is 185 kDa and clone 8H7 binds between human XPG residues Ser 947 and Ala 1165. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Jun 18 2004

    Question

    Ab46 - what size band is detected on a Western? Has the epitope been determined? Ab6264 - Has the epitope been determined?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2004

    Answer

    I am still waiting for details regarding ab46 and will contact you when I receive them. For ab6264, the originator has informed me that the epitope has not been determined.

    Read More

    Abcam Scientific Support

    回复于 Jun 17 2004

    Question

    Has this antibody been tested on paraffin sections? If so what antigen retrieval was used, and what dilutions would you recommend?

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2002

    Answer

    This antibody has not been tested on paraffin sections.

    Read More

    Abcam Scientific Support

    回复于 May 23 2002

    Question

    Has this antibody been tested in mouse?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 18 2001

    Answer

    This antibody has not yet been tested in mouse.

    Read More

    Neil Hayward

    Abcam Scientific Support

    回复于 Jun 25 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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