Anti-XPD抗体[4G2-2A6] (ab54676)
![Western blot - Anti-XPD antibody [4G2-2A6] (ab54676)](https://www.abcam.cn/ps/products/54/ab54676/Images/ab54676-67645-anti-xpd-antibody-western-blot.jpg "Western blot - Anti-XPD antibody [4G2-2A6] (ab54676)")
Key features and details
- Mouse monoclonal [4G2-2A6] to XPD
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-XPD抗体[4G2-2A6]
参阅全部 XPD 一抗 -
描述
小鼠单克隆抗体[4G2-2A6] to XPD -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein, corresponding to amino acids 1-406 of Human XPD
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常规说明
This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40 -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
4G2-2A6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54676于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 87 kDa.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 87 kDa. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
靶标
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功能
ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. -
疾病相关
Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D) [MIM:278730]; also known as XP group D (XPD). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-D patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Defects in ERCC2 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
Defects in ERCC2 are the cause of cerebro-oculo-facio-skeletal syndrome type 2 (COFS2) [MIM:610756]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
序列相似性
Belongs to the helicase family. RAD3/XPD subfamily.
Contains 1 helicase ATP-binding domain. -
翻译后修饰
ISGylated. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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数据库链接
- Entrez Gene: 2068 Human
- Omim: 278730 Human
- SwissProt: P18074 Human
- Unigene: 487294 Human
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别名
- TFIIH 80 kDa subunit antibody
- Basic transcription factor 2 80 kDa subunit antibody
- BTF2 p80 antibody
see all
图片
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Western blot - Anti-XPD antibody [4G2-2A6] (ab54676)
XPD antibody (ab54676) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Immunoprecipitation - Anti-XPD antibody [4G2-2A6] (ab54676)](https://www.abcam.cn/ps/products/54/ab54676/Images/ab54676-67644-anti-xpd-antibody-immunoprecipitation.jpg)
Immunoprecipitation - Anti-XPD antibody [4G2-2A6] (ab54676)XPD was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Mouse monoclonal to XPD and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54676.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 150kDa: SMC1; Non specific - 41 and 42kDa: We are unsure as to the identity of this extra band.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-XPD antibody [4G2-2A6] (ab54676)](https://www.abcam.cn/ps/products/54/ab54676/Images/ab54676-67643-anti-xpd-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-XPD antibody [4G2-2A6] (ab54676)Overlay histogram showing HeLa cells stained with ab54676 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54676, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (18)
ab54676 被引用在 18 文献中.
- Dai W et al. XPA Enhances Temozolomide Resistance of Glioblastoma Cells by Promoting Nucleotide Excision Repair. Cell Transplant 31:9636897221092778 (2022). PubMed: 35536165
- Bassett J et al. Systematic mutagenesis of TFIIH subunit p52/Tfb2 identifies residues required for XPB/Ssl2 subunit function and genetic interactions with TFB6. J Biol Chem 298:102433 (2022). PubMed: 36041630
- van Toorn M et al. Active DNA damage eviction by HLTF stimulates nucleotide excision repair. Mol Cell 82:1343-1358.e8 (2022). PubMed: 35271816
- Jian J et al. XPD inhibits cell growth and invasion and enhances chemosensitivity in esophageal squamous cell carcinoma by regulating the PI3K/AKT signaling pathway. Int J Mol Med 46:201-210 (2020). PubMed: 32377720
- Maio N et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
