Anti-XPA抗体(ab180618)
Key features and details
- Rabbit polyclonal to XPA
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-XPA抗体
参阅全部 XPA 一抗 -
描述
兔多克隆抗体to XPA -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human XPA aa 1-273.
Sequence:MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSA TAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEF DYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLL KDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEAL EEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEE NLEDDMYRKTCTMCGHELTYEKM
Database link: P23025 -
阳性对照
- HEK293 cell line extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/500 - 1/2000. Predicted molecular weight: 31 kDa.
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ICC/IF |
1/50 - 1/100.
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说明 |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 31 kDa. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. -
组织特异性
Expressed in various cell lines and in skin fibroblasts. -
疾病相关
Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders. -
序列相似性
Belongs to the XPA family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by HERC2 leading to degradation by the proteasome. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7507 Human
- Entrez Gene: 298074 Rat
- Omim: 611153 Human
- SwissProt: P23025 Human
- Unigene: 654364 Human
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别名
- DNA repair protein complementing XP A cells antibody
- DNA repair protein complementing XP-A cells antibody
- DNA repair protein complementing XPA cells antibody
see all
图片
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Anti-XPA antibody (ab180618) at 1/500 dilution + HEK293 cell line extract
Predicted band size: 31 kDa -
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using ab180618 Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling XPA with ab180618 at 1/200. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling XPA with ab180618 at 1/200. Magnification: 200x.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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