重组Anti-Xanthine Oxidase抗体[EPR4606] (ab133268)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4606] to Xanthine Oxidase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Xanthine Oxidase抗体[EPR4606]
参阅全部 Xanthine Oxidase 一抗 -
描述
兔单克隆抗体[EPR4606] to Xanthine Oxidase -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Xanthine Oxidase aa 1200-1300. The exact sequence is proprietary.
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阳性对照
- WB: Human milk and liver lysates, Mouse and Rat heart lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4606 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133268于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).
For unpurified use at 1/1000 - 1/10000. |
说明 |
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WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa). For unpurified use at 1/1000 - 1/10000. |
靶标
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功能
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). -
组织特异性
Detected in milk (at protein level). -
疾病相关
Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. -
序列相似性
Belongs to the xanthine dehydrogenase family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 FAD-binding PCMH-type domain. -
翻译后修饰
Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). -
细胞定位
Cytoplasm. Peroxisome. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7498 Human
- Entrez Gene: 22436 Mouse
- Entrez Gene: 497811 Rat
- Omim: 607633 Human
- SwissProt: P47989 Human
- SwissProt: Q00519 Mouse
- SwissProt: P22985 Rat
- Unigene: 250 Human
see all -
别名
- Xanthine dehydrogenase antibody
- Xanthine dehydrogenase/oxidase antibody
- Xanthine oxidase antibody
see all
图片
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All lanes : Anti-Xanthine Oxidase antibody [EPR4606] (ab133268) at 1/1000 dilution (Purified)
Lane 1 : Human milk lysate
Lane 2 : Human liver lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat heart lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 146 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab133268 被引用在 10 文献中.
- Zhou Y et al. A Randomized Trial on Resveratrol Supplement Affecting Lipid Profile and Other Metabolic Markers in Subjects with Dyslipidemia. Nutrients 15:N/A (2023). PubMed: 36771199
- Monks J et al. Perilipin-2 promotes lipid droplet-plasma membrane interactions that facilitate apocrine lipid secretion in secretory epithelial cells of the mouse mammary gland. Front Cell Dev Biol 10:958566 (2022). PubMed: 36158190
- Park JW et al. Control of rat muscle nitrate levels after perturbation of steady state dietary nitrate intake. Nitric Oxide 109-110:42-49 (2021). PubMed: 33713800
- Park JH et al. Nitric oxide attenuated transforming growth factor-ß induced myofibroblast differentiation of human keratocytes. Sci Rep 11:8183 (2021). PubMed: 33854158
- Park JW et al. Potential roles of nitrate and nitrite in nitric oxide metabolism in the eye. Sci Rep 10:13166 (2020). PubMed: 32759980
- Cortés-Puch I et al. Inhaled nebulized nitrite and nitrate therapy in a canine model of hypoxia-induced pulmonary hypertension. Nitric Oxide 91:1-14 (2019). PubMed: 31299340
- Park JW et al. Compensatory mechanisms in myoglobin deficient mice preserve NO homeostasis. Nitric Oxide 90:10-14 (2019). PubMed: 31173908
- Wylie LJ et al. Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise. J Physiol 597:5565-5576 (2019). PubMed: 31350908
- Kimball AS et al. The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair. Immunity 51:258-271.e5 (2019). PubMed: 31350176
- Li XQ et al. Liquiritin suppresses UVB-induced skin injury through prevention of inflammation, oxidative stress and apoptosis through the TLR4/MyD88/NF-?B and MAPK/caspase signaling pathways. Int J Mol Med 42:1445-1459 (2018). PubMed: 29901082