Anti-XAB2抗体(ab129487)
Key features and details
- Rabbit polyclonal to XAB2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-XAB2抗体
参阅全部 XAB2 一抗 -
描述
兔多克隆抗体to XAB2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Chinese hamster, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 805-855 of Human XAB2 (NP_064581.2).
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阳性对照
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129487于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 100 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 100 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing. -
序列相似性
Belongs to the crooked-neck family.
Contains 14 HAT repeats. -
细胞定位
Nucleus. Detected in the splicing complex carrying pre-mRNA. - Information by UniProt
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数据库链接
- Entrez Gene: 528991 Cow
- Entrez Gene: 484995 Dog
- Entrez Gene: 56949 Human
- Entrez Gene: 67439 Mouse
- Entrez Gene: 100525328 Pig
- Entrez Gene: 245976 Rat
- Entrez Gene: 704109 Rhesus monkey
- Omim: 610850 Human
see all -
别名
- 0610041O14Rik antibody
- adapter protein ATH 55 antibody
- Adapter protein ATH-55 antibody
see all
图片
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Western blot - Anti-XAB2 antibody (ab129487)All lanes : Anti-XAB2 antibody (ab129487) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-XAB2 antibody (ab129487)Lane 1: ab129487 at 0.4 µg/ml staining XAB2 in HeLa whole cell lysate immunoprecipitated using ab129487 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Detection: Chemiluminescence
Exposure time: 3 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab129487 被引用在 1 文献中.
- Sharma AB et al. XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase. Nucleic Acids Res 49:9906-9925 (2021). PubMed: 34500463